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水 (Ipomoea aquatica) 的基因改造,是一种复苏保护的多年生绿叶植物

Fayas Thayale Purayil¹, Mariam Alzaabi¹, Shina Sasi¹

¹Khalifa Center for Genetic Engineering and Biotechnology, Al Ain, United Arab Emirates.

Physiologia plantarum

|May 6, 2025

概括

这项研究优化了水 (Ipomoea aquatica) 种植的协议,使全年收获和提高粮食安全. 像Agrobacterium介导的转化等先进的技术已成功应用于作物改进.

科学领域:

植物生物技术植物生物技术
农业科学农业科学
粮食安全粮食安全

背景情况:

全球粮食安全倡议强调改善区域作物,特别是全年收获的多年叶绿菜.
水 (Ipomoea aquatica) 是一种营养丰富的,民族栽培的多年叶绿植物,对区域粮食安全至关重要.

研究的目的:

为水菜开发高效的器官生成和体质胚胎生成协议.
建立以Agrobacterium为媒介的转化和基因组编辑方法,以改善I. aquatica.
为了验证人类治疗蛋白质在转化水菜中的表达.

主要方法:

机体发育和体质胚胎发育是由各种I. aquatica扩展体 (hypocotyl,叶子,根) 诱导的.
优化的Ipomoea Basal (CLC-CP) 介质与Murashige和Skoog介质进行了比较.
使用Agrobacterium tumefaciens和Agrobacterium rhizogenes进行转换和基因组编辑 (IaNAP1).

主要成果:

在CLC-CP介质显著优于MS介质在芽生长再生和体质胚胎生成方面.
在具有特定植物生长调节剂的CLC-CP上,hypocotyl拓展剂产生了最高数量的芽 (12.4).
成功的Agrobacterium介导转化导致GFP表达的芽和胚胎;基因组编辑得到证实. 苗木在移植后显示100%的存活率.

结论:

已经建立了一个强大的协议,用于水菜的再生,转换和基因组编辑.
该协议促进了水的基因改进,以提高营养价值和可取的特征.
人类治疗性蛋白质的成功表达突出了水菜作为生物反应器的潜力.

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