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枣树中异二烯排放的高温适应与增强的基质可用性和合成酶活性降低有关

Vinícius Fernandes de Souza¹, Michelle Robin², Bahtijor Rasulov¹

¹Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu, Estonia.

Physiologia plantarum

|May 6, 2025

概括

热浪通过影响异二烯合成酶 (IspS) 和关键MEP通路中间体来增加植物异二烯排放. 适应包括二甲基酸 (DMADP) 可用性持续增加,影响未来的排放.

科学领域:

植物生理学植物生理学
生物化学生物化学
环境科学环境科学

背景情况:

植物的异二烯排放对温度敏感,但热浪反应的机制,包括酶活性和代谢适应,尚未完全理解.
异烯合成由异烯合成酶 (IspS) 和MEP通路中间体的可用性,如DMADP和MEcDP进行调节.

研究的目的:

调查IspS活动和MEP通路中间体 (DMADP,MEcDP) 在*Phoenix dactylifera*的温度过渡期间控制异烯排放中的作用.
区分瞬间的温度效应和对异烯合成的长期适应反应.

主要方法:

提交的 *Phoenix dactylifera* cv. 在这里可以查看. 在控制温度过渡 (25°C - - 37°C - - 25°C) 之间进行Medjool.
测量了DMADP和MEcDP在不同温度阶段的异二烯排放率,IspS活性和池大小.

主要成果:

温度升高最初通过增强的IspS活动增加了异二烯排放,随后由于扩大了DMADP和MEcDP池,持续增加.
由于IspS活动较低,冷却降低了低于基线的排放量,但随着基质池的补充,恢复发生了.
适应热量增加了DMADP的可用性,在冷却后仍然存在,而MEcDP的适应速度较慢,支持向DMADP的碳流.

结论:

对热浪的异二烯排放反应涉及快速的酶作用和更慢的代谢适应,主要是通过改变基质可用性.
持续的热应力可以在增加基质供应的同时抑制IspS,因此需要集成的酶动力学模型和中间池来准确预测排放.
了解温度波动下的MEP路径动态对于预测植物对气候变化的反应至关重要.

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