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Updated: May 9, 2025

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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硫酸-SDA交联的双重质谱碎片化模式

Thomas Powell1, Martin Ebner1, Andrew Creese1

  • 1Analytical Development, Immunocore Limited, Abingdon, UK.

European journal of mass spectrometry (Chichester, England)
|May 6, 2025
PubMed
概括

本研究使用各种质谱法分析交联的碎片化模式. 了解这些模式有助于确定蛋白质结构决定的交叉链接地点.

关键词:
碰撞诱导的解离是由于碰撞引起的.交叉链接是指交叉链接.通过补充HCD解离 (EThcD) 进行电子转移.分碎的分配 分碎的分配.高能碰撞引起的解离 (HCD)可光激活的交叉连接器硫酸-SDA 的使用.

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