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非编码RNAs PROX1-AS1和miR-647:在胃癌中潜在的相互作用和预后价值

Irina V Bure^1,2, Ekaterina A Vetchinkina¹, Ekaterina B Kuznetsova^1,3

¹Laboratory of Medical Genetics, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991, Moscow, Russia.

Current molecular medicine

|May 6, 2025

概括

胃癌 (GC) 的进展与特定的非编码RNA (ncRNA) 有关. 这项研究在GC组织中发现了PROX1-AS1和miR-647之间的负相关性,表明它们作为预后生物标志物的潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生化学

背景情况:

胃癌 (GC) 是全球癌症死亡的主要原因.
非编码RNAs (ncRNAs),包括microRNAs和长ncRNAs,在GC病原和进展中发挥作用.
ncRNAs是GC的潜在诊断和预后生物标志物.

研究的目的:

在GC中评估PROX1-AS1 (Prospero Homeobox 1 Antisense RNA 1) 和miR-647 (microRNA-647) 的表达.
为了研究PROX1-AS1和miR-647.7之间的相互作用.
评估它们在GC中的临床意义和预后价值.

主要方法:

使用实时聚合酶链反应 (RT-PCR) 在110个GC患者组织和65个血样本中量化了PROX1-AS1和miR-647表达.
包括38个正常的胃组织和49个健康的血样本作为对照.
分析了与临床病理特征的相关性,并进行了体外实验来评估它们的相互作用.

主要成果:

与对照组相比,PROX1-AS1表达显著下降,而miR-647表达在GC组织中增加.
在瘤和相邻的非瘤组织中观察到PROX1-AS1和miR-647之间的负相关性.
表达水平与临床病理特征相关,包括原发性瘤大小.

结论:

在GC组织和细胞系中,PROX1-AS1和miR-647之间存在负相关性.
这些ncRNAs,特别是它们与瘤大小的关联,表明GC的潜在预后价值.
对PROX1-AS1和miR-647的进一步研究可能会导致新的GC生物标志物.

关键词:

生物标志物.生物标志物.癌症细胞系是癌症细胞系中的一个.表观遗传学是指表观遗传学.胃癌胃癌是一种胃癌.长非编码RNA是什么意思这是一个微型RNA.没有编码的RNAs.

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