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Updated: May 21, 2025

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
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双步溶解增加了高灵敏度纳米HILIC/MS/MS基础蛋白质的覆盖范围.

Koshin Akamatsu1, Eisuke Kanao1,2, Ayana Tomioka1

  • 1Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan.

Analytical chemistry
|May 6, 2025
PubMed
概括

我们开发了一种两步溶解方法,以改进纳米级的水友互动色谱学,并配合双重质谱学 (nanoHILIC/MS/MS). 这种技术增强了的溶解性,并促进了蛋白质组学研究中的蛋白质识别.

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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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