相关实验视频

Updated: May 9, 2025

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration

Published on: November 26, 2015

一个具有传感元件的集成圆窗膜/耳微生理系统,用于实时研究药物反应

Jing Bai¹, Olurotimi Bolonduro¹, Pavlo Gordiichuk¹

¹Department of Living Systems, Triton Systems, Inc., Chelmsford, MA, USA. jbai@tritonsys.com.

|May 6, 2025

概括

一个新的耳机芯片模型为听力损失药物查提供了实时监控. 这种高通量系统有助于开发用于听力保护和再生的治疗方法,减少动物试验.

科学领域:

耳科技术和再生医学
用于药物发现的微生理系统.
感官器官模型的生物工程.

背景情况:

听力损失源于耳毛细胞损伤,治疗选择有限.
开发有效的治疗方法需要生理学上相关的查模型.
现有的体外模型缺乏用于内耳属性评估的复杂性.

研究的目的:

提出一种高通量微生理系统 (MPS) 用于查听力损失治疗方法.
为了实时监测内耳屏障功能和药物反应.
促进用于保护和再生听力的新药的开发.

主要方法:

一个圆窗膜 (RWM) 模型与小鼠听觉毛细胞的共同培养.
整合跨体电阻 (TEER) 传感器,用于连续监测屏障功能.
使用微流体连续流系统与石墨烯场效应晶体管 (GFET) 细胞因子传感器.

主要成果:

实时监测RWM上皮质屏障完整性和功能.
成功选保护性化合物,评估细胞活力和药物疗效 (TD50).
与商业设备和标准测试相比,验证了TEER和GFET传感器的性能.

结论:

耳上芯片MPS可实现对听力损失进行高通量药物查.
这种模型减少了对内耳损伤和再生研究的动物研究的依赖.
方便识别新的听力保护剂和再生的传递方法.

更多相关视频

A Comparative Study of Drug Delivery Methods Targeted to the Mouse Inner Ear: Bullostomy Versus Transtympanic Injection

A Comparative Study of Drug Delivery Methods Targeted to the Mouse Inner Ear: Bullostomy Versus Transtympanic Injection

Published on: March 8, 2017

Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research

Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research

Published on: June 8, 2017

相关概念视频

The Cochlea

The Cochlea

The cochlea is a coiled structure in the inner ear that contains hair cells—the sensory receptors of the auditory system. Sound waves are transmitted to the cochlea by small bones attached to the eardrum called the ossicles, which vibrate the oval window that leads to the inner ear. This causes fluid in the chambers of the cochlea to move, vibrating the basilar membrane.