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Updated: Jun 13, 2025

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使用活细胞中的蛋白质纳米进行光蛋白质的定量比较

Giulia Viola1, Yasmeen W Ibrahim1, Kyle A Jacobs1

  • 1Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94143, USA.

Journal of cell science
|May 6, 2025
PubMed
概括

mStayGold光蛋白为活哺乳动物细胞显微镜提供了卓越的性能,其功能寿命比EGFP和mEmerald长8-10倍. 这一进步有助于选择最佳的光蛋白用于细胞内生物物理分析.

关键词:
光蛋白的比较细胞内扩散的细胞内扩散.活细胞光显微镜活细胞光显微镜蛋白质纳米中的蛋白质.mScarlet 的意思是 红色的mStayGold 在黄金里停留

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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