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Updated: May 16, 2025

Fluorescence detection methods for microfluidic droplet platforms
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Fluorescence detection methods for microfluidic droplet platforms

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一种光和色度测定双模式LAMP方法用于检测Vibrio parahaemolyticus

Jinling Cui1, Yanling Wang1, Chao Jiang1

  • 1College of Chemistry and Molecular Engineering, College of Biological Engineering, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Key Laboratory of Rapid Nucleic Acid Detection, Qingdao Rapid Nucleic Acid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, P. R. China. mcp169@163.com.

Analytical methods : advancing methods and applications
|May 6, 2025
PubMed
概括

一种针对Vibrio parahaemolyticus的新双模式检测方法使用循环介导同热放大 (LAMP) 进行快速的现场病原体检测. 这种方法提供了光和视觉色度测量选项,提高了资源有限的环境中的可访问性.

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相关概念视频

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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