在缩的生长因子和高级血小板丰富的纤维素粘性骨中释放生长因子
Siniong Iao1, Xiangying Ouyang1, Jing Qiao2
1Department of Periodontology, Peking University School and Hospital of Stomatology and National Center for Stomatology and National Clinical Research Center for Oral Diseases and National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China.
来自缩生长因子 (CGF) 和高级血小板丰富纤维素 (A-PRF) 的粘性骨 (SB) 随着时间的推移缓慢释放生长因子. 先进的富含血小板纤维素的粘性骨 (A-PRF-SB) 显示出优越的生长因子释放用于骨移植.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 口腔和牙面部外科手术
背景情况:
- 缩生长因子 (CGF) 和高级富血小板纤维素 (A-PRF) 是用于再生医学的自身血缩物.
- 粘性骨 (SB) 是一种由CGF或A-PRF衍生的复合材料,旨在增强生长因子的输送.
- 了解CGF-SB和A-PRF-SB关键生长因子的释放动力学对于优化它们在骨再生中的临床应用至关重要.
研究的目的:
- 为了比较CGF-SB和A-PRF-SB的临界生长因子的体外释放模式.
- 分析血小板衍生生长因子-BB (PDGF-BB),血管内皮生长因子 (VEGF),基础纤维细胞生长因子 (bFGF),胰岛素样生长因子-1 (IGF-1) 和转化生长因子-β1 (TGF-β1) 的释放动力学.
- 根据它们的生长因子释放配置文件,确定CGF-SB和A-PRF-SB在骨移植程序中的潜力.
主要方法:
- CGF,A-PRF,CGF-SB和A-PRF-SB被化了28天时间.
- 在间隔 (1,7,14,21,28天) 收集超级.
- 使用多重试验量化PDGF-BB,VEGF和bFGF的量化;IGF-1和TGF-β1通过ELISA进行测量.
主要成果:
- 在24小时内,CGF和A-PRF表现出PDGF-BB,VEGF和bFGF的快速释放.
- CGF-SB和A-PRF-SB表现出这些增长因子的释放速度明显较慢且持续,到第7天累计释放超过50%.
- 与CGF-SB相比,A-PRF-SB显示PDGF-BB,bFGF和TGF-β1的释放显著更高 (p <0.05).与CGF-SB相比,PDGF-BB,bFGF和TGF-β1的释放显著更高 (p <0.05).
结论:
- 与单独的CGF和A-PRF相比,CGF-SB和A-PRF-SB都提供了持续的增长因素释放.
- A-PRF-SB表现出优越的生长因子释放特征,这表明骨移植应用的增强潜力.
- 粘性骨制剂的持续释放特性有利于促进骨再生.
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