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Updated: May 17, 2025

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

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使用长ssDNA和sgRNA的精确跨链交叉连接,高效精确地整合大型DNA序列

Zhigang Li1, Chengxu Li1, Shiyan Xiao1,2

  • 1Department of Polymer Science and Engineering, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China.

ACS synthetic biology
|May 6, 2025
PubMed
概括

一种新的基因编辑方法使用化学修饰的长单链DNA (lssDNA) 和光激活交叉链接来显著提高大型DNA插入的同质导向修复 (HDR) 效率和准确性.

关键词:
这就是CRISPR的敲入式.同质性导向的维修是指导维修.跨链交叉连接 跨链交叉连接异于目标效应的效应这就是Fanconi贫血修复途径.

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