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在Prop1-iCre knockin小鼠的垂体和卵巢中的重组活性的表征

Jia-Mu Zhao^1,2, Qing Han², Chunyi Chen¹

¹NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, China.

American journal of physiology. Endocrinology and metabolism

|May 6, 2025

概括

一个新的Prop1-iCre敲击小鼠系显示在垂体内分泌细胞和卵巢颗粒细胞中特定的基因重组. 这种工具有助于垂体研究,但由于卵巢活动,需要谨慎使用.

科学领域:

内分泌学在内分泌学.
发展生物学发展生物学
遗传学是一个遗传学.

背景情况:

pituitary 腺体调节重要的生理过程.
1表达的原始细胞产生了所有垂体内分泌细胞.
现有的Prop1促进器驱动的CRE小鼠已经显示出子宫外活动,使研究复杂化.

研究的目的:

开发和验证一种新的Prop1-iCre敲击小鼠系列,用于精确地对 pituitary 进行基因操纵.
评估由Prop1基因位点驱动的Cre重组酶活性的特异性和稳定性.

主要方法:

使用CRISPR/Cas9生成Prop1-iCre敲击类小鼠,以实现Prop1和Cre的双流体表达.
将Prop1-iCre小鼠与Rosa-tdTomato记者线交叉,以可视化重组.
分析CRE在各种组织中的活性,包括垂体和卵巢.

主要成果:

在所有垂体内分泌细胞中,Prop1-iCre knockin线显示出强大的和特定的重组.
在下丘脑或其他主要外围器官中没有观察到显著的Cre活性.
在卵巢颗粒细胞中也检测到重组活性,与Prop1 mRNA表达相关.

结论:

在Prop1-iCre敲击鼠标线是一个有价值的工具,用于向基因操纵在垂体内分泌细胞.
研究人员应对在卵巢颗粒细胞中观察到的重组活性保持谨慎.
与传统的Prop1促进器驱动的转基因模型相比,这一系列提供了更好的特异性.

关键词:

克雷洛克斯PPP的创作腺性低血压 (adenohypophysis) 是一种颗粒状细胞是颗粒状细胞.生殖生殖的繁殖.

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In-vitro Mutagenesis

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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