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通过集成F-ENDOR信号强度来测量19F和Gd3+之间的蛋白质和刚性规则中的纳米距离

Martyna Judd¹, Mian Qi², Elwy H Abdelkader³

¹Research School of Chemistry, Australian National University, Canberra, Australian Capital Territory 2601, Australia.

Journal of the American Chemical Society

|May 6, 2025

概括

-19 ENDOR (电子核双共振) 现在可以测量长达20 Å的蛋白质距离. 这种技术通过使蛋白质复合体中的联体位置在纳米尺度上进行三角测量来增强结构生物学.

科学领域:

结构生物学
生物物理
磁共振光谱学

背景情况:

-19 ENDOR (电子核双共振) 是测量蛋白质距离的一种有价值的技术.
目前旋转-旋转放松和线条宽度的限制使得标签间距离的测量约为15 Å.

研究的目的:

扩大-19的可测距离范围.
开发一种可靠的方法来确定蛋白质结构中较长的标签间距离.

主要方法:

使用标记有-19和偏磁标签 (Gadolinium 3+) 的几何定义良好的蛋白质规则.
整合了ENDOR频谱的强度来确定距离.
将光谱强度调整为已知的强度参考值.

主要成果:

能够测量高达20 Å的19F-Gd3+距离.
测量距离接近30 Å的指示潜力.
通过强度缩放来确定该方法的稳定性.

结论:

扩大了 19F ENDOR 在结构生物学中测量较长距离的实用性.
开发的方法允许在蛋白质-连接体内进行纳米尺度的连接体坐标三角化.

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