昂科斯塔丁-M:一种新的白细胞衍生因子,促进人类卵巢中的排卵过程
Yohan Choi1, Mats Brännström2,3, James W Akin4
1Department of Obstetrics and Gynecology, University of Kentucky College of Medicine, Lexington, KY, USA.
Human reproduction (Oxford, England)
|May 6, 2025
概括
白细胞衍生的哥斯塔丁-M (OSM) 激活了人体颗粒细胞中的关键信号通路,在排卵期间影响了类固醇生成和前列腺素合成. 这种细胞因子在调节排卵事件和卵泡成熟方面发挥着至关重要的作用.
科学领域:
- 生殖内分泌学 生殖内分泌学
- 免疫学 免疫学 免疫学
- 细胞信号传递 细胞信号传递
背景情况:
- 白细胞对于排卵至关重要,先前的研究已经确定了白细胞中的奥诺斯塔丁-M (OSM) 以及毛囊细胞中的受体.
- 在这项研究之前,人类排卵卵泡中白细胞衍生OSM的精确功能在很大程度上是未知的.
研究的目的:
- 为了研究白细胞衍生型的安哥斯塔丁-M (OSM) 是否调节人类主导卵泡中的排卵过程.
- 阐明OSM在人类排卵前粒状细胞中的作用及其对关键排卵事件的影响.
主要方法:
- 对自然循环女性和试管婴儿患者的主导卵泡的分析.
- 使用免疫组织化学和定量PCR (qPCR) 评估OSM和受体表达.
- 在体外研究中,使用初级人体颗粒素/蛋白质细胞 (hGLCs) 用复合人体OSM (rhOSM) 和hCG治疗.
- 功能性分析包括西部涂抹,RNA测序和激素测定孕激素,雌激素和前列腺素E2.
主要成果:
- OSM仅在白细胞中表达,而它的受体主要在粒状细胞中.
- rhOSM激活了hGLC中的STAT3,ERK1/2,AKT和p38MAPK信号通路.
- rhOSM调节的基因表达与类固醇生成,前列腺素合成和组织重塑有关.
- rhOSM增加了孕激素和前列腺素E2分泌,同时降低了雌激素的产生.
结论:
- 白细胞衍生OSM是一种关键的细胞因子,它调节人类卵泡中的排卵事件.
- 颗粒细胞中的OSM信号影响类固醇生成,前列腺素合成和组织重塑.
- 这些发现为卵泡成熟期间的免疫内分泌交叉反应提供了新的见解,并可能为生育治疗提供信息.
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