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Zohreh Mirveis¹, Nitin Patil¹, Hugh J Byrne²

¹FOCAS Research Institute, TU Dublin, City Campus, Camden Row, Dublin 8, Ireland; School of Physics, Optometric and Clinical Sciences, TU Dublin, City Campus, Grangegorman, Dublin 7, Ireland.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|May 6, 2025

概括

减弱总反射率-里埃变换红外光谱 (ATR-FTIR) 可以分析低代谢物度. 多变量曲线分辨率-交替最小平方 (MCR-ALS) 有效追踪糖解,但与含有谷氨的复杂混合物作斗争.

科学领域:

生物化学和光谱学生物化学和光谱学
代谢途径分析
癌症代谢研究癌症代谢研究

背景情况:

像糖溶解和谷氨酸溶解这样的代谢途径在癌症等疾病中至关重要.
在细胞外介质中分析低度的代谢物对于理解代谢转变至关重要.
减弱总反射度-里埃变换红外光谱 (ATR-FTIR) 为此类分析提供了一种潜在的方法.

研究的目的:

探索减弱总反射率-里埃变换红外光谱 (ATR-FTIR) 探测低代谢物度的量化.
应用多变量曲线分辨率-交替最小平方 (MCR-ALS) 进行数据挖掘的代谢途径动力学.
为了研究谷氨胺对糖解分析的影响.

主要方法:

使用ATR-FTIR光谱法测量细胞外介质中的葡萄糖,谷氨酸和乳酸度.
采用样本提取和在ATR晶体上干燥用于光谱分析.
应用MCR-ALS来解构细胞代谢的时间序列光谱数据.

主要成果:

通过ATR-FTIR成功地测量了低度的葡萄糖,谷氨酸和乳酸,具有强烈的吸收度相关性.
MCR-ALS从糖溶解数据中分解葡萄糖和乳酸盐,表明消耗率为0.0016分钟-1.1.
当谷氨胺与乳酸信号重叠时,MCR-ALS在解复杂混合物方面面临限制.

结论:

在代谢研究中,ATR-FTIR光谱学有效分析关键代谢物的低度.
MCR-ALS可以成功地模拟简单的代谢途径,如糖解,但对复杂的混合物有限制.
需要进一步开发方法,以应对多代谢物分析中的光谱重叠挑战.

关键词:

通过ATR-FTIR光谱学检测.葡萄糖氨基溶解是什么葡萄糖溶解是什么? 葡萄糖溶解在MCR-ALS数据分析中,分析了MCR-ALS数据.代谢途径的动力学频谱数据挖掘是指对光谱数据进行挖掘.

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