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Updated: Jun 13, 2025

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
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Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

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通过冷EM检测发现的自然装饰的RNA复合物

Rachael C Kretsch1, Yuan Wu2, Svetlana A Shabalina3

  • 1Biophysics Program, Stanford University, Stanford, CA, USA.

Nature
|May 6, 2025
PubMed
概括

研究人员确定了三种大型细菌RNA的3D结构,揭示了复杂的RNA单独纳米和二维复合体. 这些装饰丰富的RNA结构突显了它们的生物重要性和自我组装能力.

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Nanomanipulation of Single RNA Molecules by Optical Tweezers06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

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Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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