相关实验视频

Updated: May 9, 2025

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles

Published on: October 18, 2024

[悬浮的Vero细胞的转录组分析和通过表皮生长因子减少细胞自]

Muzi Li^1,2,3, Na Sun^1,2,4, Runsheng Peng^1,2,4

¹Ministry of Education Engineering Research Centre for Key Technology and Industrialisation of Cell-Based Vaccines, Northwest Minzu University, Lanzhou 730030, Gansu, China.

Sheng wu gong cheng xue bao = Chinese journal of biotechnology

|May 6, 2025

概括

高水平的自促使悬浮的Vero细胞的生长速度缓慢,活力低下. 用表皮生长因子 (EGF) 补充剂减少了自,增强了细胞生长和活力.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

悬浮的Vero细胞培养具有挑战,包括细胞活力降低和延长翻倍时间.
了解这些限制背后的分子机制对于优化细胞培养条件至关重要.

研究的目的:

研究与附着Vero细胞 (Vero-AD) 相比,悬浮Vero细胞 (Vero-XF) 增长缓慢和活力低下的原因.
评估表皮生长因子 (EGF) 补充对Vero-XF生长和自的作用.

主要方法:

转录组分析以确定Vero-XF和Vero-AD.之间的差异表达基因 (DEG).
基因和基因组的京都百科全书 (KEGG) 路径丰富分析.
定量实时PCR (qRT-PCR) 和西式涂抹用于验证基因和蛋白质的表达.
评估EGF补充后的细胞生长率和细胞亡率.

主要成果:

转录组分析显示了7,376个DEG,主要丰富在自和髓通路中.
与自相关的关键基因 (例如,ATG9B,WIPI2,LAMP2) 显示出改变的表达,LC3II/I增加和P62蛋白水平降低表明Vero-XF的自更高.
根据剂量,EGF补充剂减少了Vero-XF的自,增加了特异性生长率,并降低了亡率.

结论:

激活的自是导致悬浮Vero细胞生长不良和活力的重要因素.
皮表皮生长因子 (EGF) 通过抑制自而有效缓解这些问题,从而改善细胞培养结果.
这些发现为优化悬浮Vero细胞培养协议提供了基础.

关键词:

维罗细胞是维罗细胞.自自是自的过程.皮肤表皮生长因子悬浮细胞培养的悬浮细胞培养翻译学翻译学翻译学翻译学

更多相关视频

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

Published on: October 28, 2019

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

Published on: August 25, 2018

相关概念视频

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...