大规模的转录组采矿使得宏观循环多样化,并提高了酸脚手架的生物活性
Xiaofeng Wang1, Khadija Shafiq1, Derrick A Ousley1
1Department of Medicinal Chemistry, University of Michigan, Ann Arbor, MI, USA.
Nature communications
|May 6, 2025
概括
我们开发了一种分析植物RNA-seq数据的新方法,发现了新的moroidin化合物. 这些植物衍生具有作为对肺癌细胞的细胞毒剂的潜力.
科学领域:
- 生物化学 生物化学
- 基因组学就是基因组学.
- 药用化学 医学化学
背景情况:
- 在公共档案中存在大量的植物RNA-seq数据.
- 由于数据库的大小,搜索这些未组装的数据以寻找生物合成基因具有挑战性.
- 核糖体合成和翻译后修饰的 (RiPPs) 是一类有前途的天然产品.
研究的目的:
- 优化RNA-seq组装,为生物合成基因发现创建可搜索的数据库.
- 探索一类植物RiPPs. moroidins的多样化.
- 为了确定具有潜在药用应用的新型莫里丁类比物.
主要方法:
- 为大型公共数据集优化RNA-seq组装管道.
- 转录组挖掘用于识别参与摩洛因生物合成的基因.
- 化学分析和细胞毒性测试发现的moroidin类似物.
主要成果:
- 将大量公开的RNA-seq数据转化为可搜索的数据库.
- 发现了具有多样化的第二环结构的新型莫里丁类似物.
- 鉴定了一种来自水草的moroidin类似物,对肺腺癌细胞有增强的细胞毒性.
- 扩大了已知分布的酸类型的酸新植物家族.
结论:
- 大规模的转录组挖掘对于发现新型真核RiPPs是有效的.
- 优化的RNA-seq组件有助于生物合成基因的发现.
- 莫罗伊丁类似物是开发新抗癌疗法的有希望的途径.
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