乳球植物菌植物外细胞囊泡通过STING依赖的自产生抗PEDV效应
Shihan Zhang1,2,3, Xin Shan1,2,3, Guanglong Qu1,2,3
1Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Changchun, 130118, China.
BMC microbiology
|May 6, 2025
概括
乳植物菌植物细胞外囊泡 (LpEVs) 激活猪细胞中的cGAS-STING通路和自,增强对猪流行性腹病毒 (PEDV) 的抗病毒免疫力. LpEVs显示出显著的PEDV抑制,突出显示了它们作为抗病毒剂的潜力.
科学领域:
- 兽医免疫学 兽医免疫学
- 微生物学 微生物学
- 细胞生物学 细胞生物学
背景情况:
- 猪流行性腹病毒 (PEDV) 在猪产业中造成重大经济损失.
- 通过细胞外囊泡 (EV) 输送的益生菌可以调节肠道上皮细胞中的先天免疫力.
- cGAS-STING通路和自对抗病毒防御和细胞平衡至关重要.
研究的目的:
- 为了研究Lactiplantibacillus plantarum细胞外囊泡 (LpEVs) 对PEDV感染的抗病毒作用.
- 阐明cGAS-STING信号通路和自在LpEV介导的抗病毒反应中的作用.
- 确定LpEVs的最佳度,以抑制PEDV的扩散.
主要方法:
- 猪肠上皮细胞的治疗 (IPEC-J2) 与不同度的LpEVs.
- 对cGAS-STING通路激活和I型干扰素诱导的分析.
- 对自水平和STING依赖性的评估.
- 量化LpEVs对PEDV复制抑制的量化.
主要成果:
- 在IPEC-J2细胞中,LpEVs显著激活了cGAS-STING通路,增强了抗病毒免疫力.
- 观察到一种潜在的反调节机制,较低的LpEV度显示出更高的cGAS-STING激活功效.
- LpEVs诱导了STING依赖的自,这与增强的PEDV抑制有关,特别是在10μg/mL时.
- 所有测试的LpEV度 (2.5,5和10微克/毫升) 都抑制了PEDV的扩散,其中10微克/毫升显示出最强的效果.
结论:
- 通过激活cGAS-STING通路并诱导自,LpEVs可以增强对PEDV的抗病毒先天免疫力.
- LpEVs的抗病毒活性依赖于度,并与自诱导有关.
- LpEVs代表了一种有前途的治疗剂,用于预防猪中PEDV感染.
相关概念视频
Pinching-off of Coated Vesicles
2.9K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
2.9K
Receptor-mediated Endocytosis
5.8K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
5.8K
Receptor Downregulation in MVBs
2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.0K
Intralumenal Vesicles and Multivesicular Bodies
3.3K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.3K


