相关实验视频

Updated: May 23, 2025

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
16:11

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Published on: September 16, 2013

63.6K

解密转录-翻译-折叠 (TX-TL-FD) 用于增强T7系统和配备遗传护卫Escherichia coli菌株中的丁酶生产

Chuan-Chieh Hsiang1, I-Son Ng1

  • 1Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.

ACS synthetic biology
|May 7, 2025
PubMed
概括

优化转录 (TX) 和翻译 (TL) 是使用T7RNA聚合酶 (T7RNAP) 系统在工程化大肠杆菌中高水平蛋白质生产的关键. 协调的TX-TL调节显著提高了具有挑战性的酶的表达.

关键词:
埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.在T7RNA聚合酶中,陪伴者是一个陪伴者.关键词: 关键词: 关键词: 关键词: 关键词: 关键词: 关键词:转录 转录 是一种转录.翻译启动地区地区

更多相关视频

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase09:31

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Published on: September 26, 2020

4.2K
Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs08:24

Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs

Published on: November 19, 2020

3.3K

相关概念视频

Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.1K
Molecular Chaperones and Protein Folding03:00

Molecular Chaperones and Protein Folding

The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein.
The...
17.6K