特拉斯皮阿文塞抑制了与肠道微生物群相关的TNF炎症通路,以缓解性结肠炎
Wenkai Wang1, Yiyang Zhao1, Ziwei Wang1
1Department of Oncology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Frontiers in immunology
|May 7, 2025
概括
拉斯皮阿文斯 (TA) 通过调节肠道微生物群和抑制炎症,有效治疗性结肠炎 (UC). 它的活性化合物伊索维素直接保护肠道细胞,为UC管理提供了多目标的方法.
科学领域:
- 药理学 药理学是指药理学的学科.
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
背景情况:
- 拉斯皮阿文斯 (TA) 是一种传统的中国草药,用于治疗性结肠炎 (UC).
- 对于UC的TA的精确治疗机制在很大程度上仍未被阐明.
- 这项研究研究了TA在UC治疗中的疗效和分子机制.
研究的目的:
- 评估TA对硫酸 (DSS) 诱导的UC的治疗作用.
- 阐明TA的机制,包括肠道微生物群调节和炎症通路调节.
- 识别和验证UC治疗中TA的活性成分和分子标.
主要方法:
- 在小鼠中使用DSS诱导的UC模型,肠道微生物群分析 (16SrRNA测序),网络药理学,分子对接和体外/体内验证.
- 对炎症性细胞因子,氧化应激标记物和肠道屏障完整性的分析.
- 肠道微生物群和宿主炎症反应之间的相关性分析.
主要成果:
- 剂量依赖的TA缓解了UC症状,保持了肠道屏障的完整性,并抑制了微生物转移.
- TA调节肠道微生物群的组成,减少病原性细菌,如埃舍里希亚-希格拉.
- 网络药理学确定了TNF-α和IL-6作为关键标,而异素对TNF-α具有很高的亲和力,通过体外和体内实验验验证.
结论:
- TA通过协同机制改善UC:肠道微生物群重塑,TNF-α/NF-κB通路抑制和肠道屏障保护.
- 作为一种活性TA成分的异维毒素,直接减轻TNF-α诱导的上皮损伤.
- 这些发现支持TA的UC多目标药理学,并建议异素作为潜在的精确治疗剂.
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