来自ICU环境的空气中的颗粒物中的可转移抗生素耐药性等离子体的表征
Kexing Zhang1,2, Xumei Zhou2, Xu Zhang3
1School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China.
iScience
|May 7, 2025
概括
在重症监护室 (ICU) 空气中发现的可转移抗生素耐药性等离子体 (TARP) 可以传播抗生素耐药性基因. 这些空气传播的TARP可能会导致耐药细菌菌株的出现.
科学领域:
- 环境微生物学环境微生物学
- 分子生物学分子生物学
- 公共卫生 公共卫生
背景情况:
- 重症监护室 (ICU) 是抗生素耐药细菌的热点.
- 可转移抗生素耐药性等离子体 (TARP) 推动了抗生素耐药性基因 (ARG) 的传播.
- 在ICU中空中TARP的存在和作用仍未得到充分研究.
研究的目的:
- 调查TARP在ICU空中环境中的存在和特征.
- 为了识别这些空中TARP所携带的抗生素耐药性基因和毒性因素.
- 了解空气TARPs对ICU中抗生素耐药性传播的潜在贡献.
主要方法:
- 从ICU空气样本中直接分离出耐药性等离子体,使用*Escherichia coli* CV601作为受体.
- 等离子体类型,测序用于识别ARG和毒性因子.
- 耐药性表型的验证和TARP的遗传学分析.
主要成果:
- 在ICU空气中检测到30种不同的等离子体类型,其中IncX3是最常见的.
- 确定了245个ARG,包括主导基因,如*bla*NDM-53,*bla*SHV-12和*BRP(MBL) *.
- 遗传学分析表明,已识别的TARP来源于通常殖民人类粘膜的细菌.
结论:
- 密切护理的空中TARP是ARG的潜在储存库.
- 空气中的TARPs可以促进抗生素耐药性的传播.
- 这些发现强调了监测ICU中的空气污染物的重要性,以有效控制感染.
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