一种基于FPGA的低成本方法,用于堆积校正的高速in vivoFLIM成像
Felipe Velasquez Moros1,2, Dorian Amiet3, Rachel M Meister1,2
1University of Zurich, Institute of Pharmacology and Toxicology, Zurich, Switzerland.
Neurophotonics
|May 7, 2025
概括
我们开发了一种新方法,称为激光周期盲时间 (LPBT),用于修复光终身成像 (FLIM) 显微镜中的扭曲. 这使得神经科学研究中的实时成像更快,更准确.
科学领域:
- 神经科学是一个神经科学.
- 生物光子学 生物光子学
- 定量成像技术 定量成像技术
背景情况:
- 基于强度的双光子显微镜在量化神经科学研究中的度测量方面是有限的.
- 使用与时间相关的单光子计数 (TCSPC) 的光寿命成像 (FLIM) 提供了定量测量,但在高速时受到"堆积"扭曲的阻碍.
研究的目的:
- 引入一种新的方法",激光周期盲时间" (LPBT),用于纠正TCSPC-FLIM中的堆积扭曲.
- 为了实现可靠,低成本的TCSPC-FLIM在高光子计数率提高神经科学研究.
主要方法:
- 开发和评估LPBT方法,使用TCSPC数据收集的in silico模拟.
- 在低成本的现场可编程网关阵列 (FPGA) 硬件上实现了LPBT校正.
- 通过体外,体外和体内实验验证实了该方法.
主要成果:
- 该LPBT方法纠正堆积扭曲,实现高精度的生命周期测量.
- 启用TCSPC-FLIM,计数速度超过传统限制的10倍以上.
- 促进了具有亚细胞分辨率的次秒代谢物动态的强大的FLIM成像.
结论:
- 该LPBT方法提供高精度,具有成本效益的FLIM成像,并显著提高采集速度.
- 这一进步有助于更广泛地采用FLIM用于神经科学和其他研究领域的定量实时成像.
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