Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Overview of Exosomes01:36

Overview of Exosomes

2.7K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Inflammatory phenotypes in acute respiratory distress syndrome: external validation and model simplification in multicenter cohorts.

Frontiers in medicine·2026
Same author

Naturally occurring mutations in replication proteins of a small RNA virus that alter the number, sizes, and relative abundances of subgenomic RNAs.

PLoS pathogens·2026
Same author

Association of serum S100A8/A9 with sepsis-associated acute kidney injury and 28-day mortality: a hybrid cohort study.

Scientific reports·2026
Same author

Multi-Modal Characterization of the Interactions between a Recombinant Protein Antigen and a Liposomal Adjuvant.

Molecular pharmaceutics·2026
Same author

FTL008.16, a 5T4-Conditional 4-1BB bispecific antibody, potently enhances antitumor immunity via tumor-directed t-cell activation.

Oncoimmunology·2026
Same author

A synchronous counter-positive strategy via online CE-SELEX for high-efficiency aptamer selection.

Journal of chromatography. A·2026

相关实验视频

Updated: May 12, 2025

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
09:30

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

7.3K

[对外瘤向的选技术的进步]

Li-Ting Zheng1, Ge Yang2, Feng Qu1

  • 1School of Life Science, Beijing Institute of Technology, Beijing 100081, China.

Se pu = Chinese journal of chromatography
|May 7, 2025
PubMed
概括

核酸体为检测外体细胞提供了一个有前途的工具,外体细胞是早期疾病诊断的关键生物标志物. 本综述详细介绍了aptamer选择方法,以克服在临床应用中识别特定外体的挑战.

科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 纳米技术纳米技术

背景情况:

  • 外体对细胞间通信和疾病生物标志物发现至关重要.
  • 开发敏感的外体检测方法对于早期诊断和治疗至关重要.
  • 核酸体是具有治疗和诊断潜力的高度特异性的结合分子.

研究的目的:

  • 对外体检测的aptamer选择策略和选技术进行审查.
  • 讨论各种外体-体查方法的优点和局限性.
  • 要突出挑战和未来的方向在外体细胞体的发展.

主要方法:

  • 通过指数式丰富 (SELEX) 系统地演化配体,用于体生成.
  • 详细审查基于SELEX的五种关键选技术:磁珠-SELEX,微流体-SELEX,纤维素-SELEX,细胞-SELEX和毛细血管电泳-SELEX.
  • 分析每个选方法的分离原则,好处,缺点和应用.

主要成果:

  • 在诊断和治疗方面,aptamers显示出对外体向的巨大潜力.
  • 五种不同的基于SELEX的方法提供了对外体细胞的查不同方法.
  • 由于外体组复杂性和异质性,在体选择方面仍然存在挑战.
关键词:
一个aptamer的应用程序.外基因组是外基因组中的一个.通过指数式丰富 (SELEX) 的联体的系统演化.

更多相关视频

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
10:46

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

3.4K
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.3K

相关实验视频

Last Updated: May 12, 2025

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
09:30

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

7.3K
A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
10:46

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

3.4K
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.3K

结论:

  • 有效的阿普坦体查技术对于推进基于外体组的诊断至关重要.
  • 需要进一步研究新的查方法,以克服外体组异质性.
  • 优化的阿帕特默选择将提高临床应用的外体检测.