使用硫版本的o-氨基三酸盐 (APTRA) 增强Zn2+的结合:引入S-APTRA和衍生物
Christopher Hogg1, Laura L Duncan1, David Parker1
1Department of Chemistry, Durham University, South Road, Durham DH1 3LE, U.K.
Inorganic chemistry
|May 7, 2025
概括
一个新的含硫的配体,S-APTRA,选择性地与高亲和力结合离子 (Zn2+). 这一发现推动了对生物学上重要的金属离子的新型传感器的开发.
科学领域:
- 协调化学 协调化学
- 超分子化学 超分子化学
- 分析化学 分析化学
背景情况:
- 奥尔托-阿米诺-N,N,O-三酸盐 (APTRA) 是一种用于Mg2+传感的五牙联体.
- APTRA对Ca2+和Zn2+的亲和力比Mg2+更高.
- 选择性金属离子连接体的开发对于生物传感应用至关重要.
研究的目的:
- 合成和描述APTRA的硫类比物,命名为S-APTRA.
- 为了研究S-APTRA及其类型的金属离子结合特性.
- 探索这些新配体在开发传感器方面的潜力.
主要方法:
- 合成S-APTRA及其四牙类型的S-APDIA.
- 紫外线吸收光谱用于监测缓冲水溶液中的金属离子结合.
- 氧化S-APTRA和S-APDIA到它们相应的硫氧化物 (SO-APTRA,SO-APDIA).
主要成果:
- 在Mg2+和Ca2+上,S-APTRA显示出对Zn2+ (Kd = 6.6 ± 0.3 nM) 的高选择性和亲和力.
- 牙酸类型的S-APDIA对Zn2+的亲和力较低 (Kd = 8 ± 1 μM).
- 氧化衍生物SO-APTRA和SO-APDIA表现出较弱的Zn2+结合亲和力.
结论:
- 在APTRA中用硫代替氧气可以提高Zn2+的选择性,与硬和软酸和基原则保持一致.
- S-APTRA及其衍生品代表了基于碳酸盐的传感器的有前途的新类联结体.
- 这些配体的可调节的结合亲属性为各种传感器应用提供了潜在的潜力.
更多相关视频
11:04Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
Published on: September 7, 2019
9.1K
13:04Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
Published on: May 16, 2019
38.5K
相关概念视频
01:19Complexation Equilibria: The Chelate Effect
416
In complexation reactions, metal atoms or cations interact with ligands to form donor-acceptor adducts called metal complexes. Ligands that bind through one donor site are monodentate, ligands with two donor sites are bidentate, and those with more than two donor sites are polydentate ligands. For example, ethylene diamine is a bidentate ligand that binds through two nitrogen donor atoms, forming a five-membered ring. EDTA is a polydentate ligand that binds through four oxygen and two nitrogen...
416
02:51Metal-Ligand Bonds
20.4K
The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
20.4K
00:56Extraction: Advanced Methods
398
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
398
01:09Complexation Equilibria: Factors Influencing Stability of Complexes
316
In complexation reactions, metal cations are the electron pair acceptors, and the ligands are the electron pair donors. The stability of the metal complexes depends primarily on the complexing ability of the central metal ion and the nature of the ligands. Generally, the complexing ability of the metal ion depends on the size and charge of the ion. As the metal ion size increases, the stability of the metal complexes decreases, provided that the valency of the metal ion and the ligands remain...
316
00:43Complexometric Titration: Ligands
869
Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...
869
00:49Ligand Binding and Linkage
4.7K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.7K