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EXPRESS:脊柱投射神经元对 Tacr1 和 Gpr83 的表达

Wenhui Ma¹, Allen Dickie¹, Erika Polgár¹

¹School of Psychology and Neuroscience, Sir James Black Building, University of Glasgow, Glasgow, G12 8QQ, UK.

|May 7, 2025

概括

这项研究揭示了疼痛感应前侧系统 (ALS) 神经元在表面背角表达Tacr1和Gpr83受体的显著重叠. 这些神经元投射到VPL丘脑,有助于疼痛感知.

科学领域:

神经科学是一个神经科学.
疼痛研究疼痛研究
身体的感觉 (somatosensation) 是一种体内感觉.

背景情况:

前侧神经系统 (ALS) 神经元传递疼痛,和温度信号.
之前的研究表明,基于Tacr1和Gpr83的表达,ALS神经元的不同群体具有有限的重叠和没有VPL投影.
这些发现与已知关于神经素1受体 (NK1r) 表达和VPL连接性的已知知识形成对比.

研究的目的:

重新评估Tacr1和Gpr83在小鼠表面背角 (SDH) ALS神经元中的表达模式.
为了澄清表达这些G蛋白结合受体 (GPCRs) 的ALS神经元的投影目标.
关于ALS神经元异质性和胸膜连接性的相互矛盾数据的协调.

主要方法:

使用了 Tacr1CreERT2 和 Gpr83CreERT2 的小鼠模型.
使用逆行和逆行神经元追踪技术.
分析了表面背角的受体表达和轴突投射模式.

主要成果:

大约90%的SDH ALS神经元表达Tacr1,而40-50%的神经元表达Gpr83.
在表达TACR1和Gpr83的ALS神经元群体之间存在显著的重叠.
来自Tacr1和Gpr83表达ALS神经元的轴突成功地投射到腹后侧 (VPL) 乳头核.

结论:

与之前的报道相反,Tacr1和Gpr83在相当一部分SDH ALS神经元中共同表达.
表达这些GPCRs的ALS神经元向VPL丘脑发射,这是传感信息的关键中继器.
这些发现表明,ALS神经元群体的重叠有助于通过VPL投影来处理疼痛的感觉歧视方面.

关键词:

前两侧的系统.泰拉木斯体的VPL核侧面的双臂部区域.神经基因素1受体的受体后突触的背脊柱路径.脊髓支柱体管道中的脊柱体管道.

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