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Updated: May 16, 2025

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell
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Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Published on: August 4, 2015

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从光时间序列中结合动力学的贝叶斯推理

J Shepard Bryan1,2, Stanimir Asenov Tashev3,4, Mohamadreza Fazel1,2

  • 1Department of Physics, Arizona State University, Tempe, Arizona 85281, United States.

The journal of physical chemistry. B
|May 7, 2025
PubMed
概括

这项研究引入了一种新方法,可以准确地测量光数据的结合和解结合率,即使有噪音和光漂白. 该方法可靠地推断出运动速率与光漂白一起,改善结合动力学分析.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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The Equilibrium Binding Constant and Binding Strength

The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
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