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Updated: Jul 2, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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一个与CRISPR/Cas12a合的多重放大系统,用于对miRNA-155的超敏感检测

Zhengdong Ai1, Wenjun Wang1, Xiang Li1

  • 1School of Basic Medical Sciences, Southwest Medical University, Luzhou 646000, P. R. China. zhousy@swmu.edu.cn.

Analytical methods : advancing methods and applications
|May 7, 2025
PubMed
概括

一个新的CRISPR/Cas12a平台使敏感的微RNA (miRNA) 检测成为可能. 该系统集成了多重放大,用于在复杂样本中快速识别视觉疾病生物标志物.

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相关概念视频

CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...