牛乳杆菌 helveticus HY7804 调节肠肝轴以改善与代谢功能障碍相关的脂肪性肝病在小鼠模型中
Hyeonji Kim1, Hye-Jin Jeon1, Ji-Woong Jeong1
1R&BD Center, hy Co., Ltd., 22, Giheungdanji-ro 24beon-gil, Giheung-gu, Yongin-si 17086, Republic of Korea.
乳酸菌 helveticus HY7804 (HY7804) 通过增强肠道屏障功能和调节肠道微生物群来改善与代谢功能障碍相关的脂肪性肝病 (MASLD). 这种益生菌可以缓解MASLD的症状,促进肠道健康.
科学领域:
- 胃肠病学和肝病学
- 微生物学和免疫学
- 营养科学 营养科学
背景情况:
- 与代谢功能障碍相关的脂肪性肝病 (MASLD) 是一个普遍存在的全球健康问题.
- 之前的研究表明,在MASLD模型中,Lactobacillus helveticus HY7804 (HY7804) 可以抑制肝脏脂肪生成,炎症和纤维化.
- 需要进一步研究HY7804在恢复肠道屏障功能和调节MASLD中的肠道微生物群中的作用.
研究的目的:
- 评估Lactobacillus helveticus HY7804 (HY7804) 在改善MASLD症状方面的疗效.
- 评估HY7804对MASLD肠道屏障完整性和肠道微生物群组成的影响.
- 阐明HY7804对MASLD和肠道健康的有益影响背后的分子机制.
主要方法:
- 通过特定的饮食诱导小鼠使用MASLD,持续7周.
- 小鼠每天接受口服HY7804 (10^9 CFU/kg/天),类型菌株或皮奥格利塔 (阳性对照) 的剂量.
- 分析了生理和生化指标,组织学分数,基因表达 (脂生,亡,紧接口,炎症) 和肠道微生物群组成.
主要成果:
- HY7804显著缓解了MASLD的生理和血液生物化学指标,并降低了组织学活性得分.
- HY7804通过TLR4/MyD88/NF-κB通路调节了与脂肪酸氧化和紧密结合完整性相关的基因,同时通过TLR4/MyD88/NF-κB通路调节了与亡相关的基因并抑制了肠道炎症.
- HY7804调节肠道微生物群,增加有益的细菌 (Bacteroidaceae, Bacteroides) 和减少有害的细菌 (Clostridiaceae,Clostridium,Streptococcaceae,Lactococcus,Lachnospiraceae),与改善的肠道健康和MASLD症状相关.
结论:
- 在小鼠模型中,Lactobacillus helveticus HY7804在缓解MASLD症状和改善肝脏健康方面表现出显著的有效性.
- HY7804通过调高紧密结基因表达来增强肠道屏障功能,并减少炎症.
- HY7804作为肠道微生物群的有益调节剂,表明其作为MASLD管理和改善肠道健康的功能补充剂的潜力.
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