在Trichoderma sp.中优化和生物反应器扩大细胞酶生产. KMF006用于更高的产量和性能
Seongwoo Myeong1, Yun-Yeong Lee1, Jeonghee Yun1
1Department of Forest Products and Biotechnology, Kookmin University, Seoul 02707, Republic of Korea.
International journal of molecular sciences
|May 7, 2025
概括
优化浸泡发酵 (SmF) 条件,包括3:1的Avicel:纤维素比率和180rpm的动与流,显著提高了Trichoderma sp.的纤维素酶产量. KMF006用于工业应用.
科学领域:
- 生物技术是生物技术.
- 酶工程是什么? 酶工程是什么?
- 工业微生物学 工业微生物学
背景情况:
- 细胞酶的生产对于生物燃料和生物化学工业至关重要.
- 优化浸泡发酵 (SmF) 参数是提高酶产量和经济可行性的关键.
- 三皮虫 sp. 这种虫. 是工业相关酶的成熟生产商.
研究的目的:
- 使用Trichoderma sp.优化操作参数,以增强使用Trichoderma sp.的细胞酶生产. 在SmF中KMF006.
- 评估10L生物反应器中优化条件的扩展可行性.
- 研究基质组成,动,流和生物炭对细胞质产量的协同效应.
主要方法:
- 阿维塞尔的瓶尺度优化:纤维素比率,动速度 (150-210rpm) 和流 (混式与非混式瓶).
- 将优化条件应用于10升生物反应器,评估螺旋引发的流.
- 对细胞酶活动 (EG,BGL,CBH) 和纸单元 (FPU) 的分析.
主要成果:
- 3:1的飞:纤维素比率 (A3C1) 和180rpm的动加速了生产,并且是最佳的.
- 流,特别是在混的瓶中,显著增加了内葡萄糖酶 (EG),β-葡萄糖酶 (BGL) 和细胞核酶 (CBH) 的活性.
- 生物炭的添加显示了与流的协同效应,进一步增强了细胞酶的产生.
- 在A3-180_Imp条件下的10L生物反应器中,扩大规模实现了84FPU/mL,增加了两倍,但过度的流 (210rpm) 降低了酶稳定性.
结论:
- 优化的SmF条件,平衡基质比率,和流,对于高效的工业规模的纤维化生产至关重要.
- 水力动力学条件在最大限度地提高酶产量和活性方面发挥着重要作用.
- 对切割应力管理的进一步研究是必要的,以便在更高的流水平下保持酶稳定性.
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