在慢性鼻炎中识别新型黄金葡萄球菌核心和辅助毒性模式
Simon P Goldie1,2, Laurie C Lau1, Huw A S Jones2
1School of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton SO16 6YD, UK.
International journal of molecular sciences
|May 7, 2025
概括
黄金葡萄球菌的毒性因子在健康人群和慢性鼻炎患者之间有所不同. 黄金菌中的特定基因模式可能会导致鼻腔持续感染和抗生素耐药性.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
背景情况:
- 黄金葡萄球菌 (S. aureus) 的鼻腔定居在健康个体和慢性鼻炎 (CRS) 患者中很普遍.
- 耐治疗的金黄色细菌生物膜和细胞内持久性是CRS的标志,需要特定的毒性因子基因表达.
- 在S. aureus分离物中,明显的毒性因子概况可能有助于CRS患者的持久性和细胞内生存.
研究的目的:
- 为了研究和比较S. aureus的毒性因子基因模式,从非患病的对照,没有鼻息肉的CRS (CRSsNP) 和带鼻息肉的CRS (CRSwNP) 患者中分离出来.
- 在CRS的背景下,确定与S. aureus持久性和细胞内生存相关的特定毒性因素.
- 探索S. aureus病毒性因子集群与CRS中的抗生素耐药性之间的潜在联系.
主要方法:
- 从77名个体的鼻子拭子中获得的S. aureus分离物的全基因组测序 (对照组,CRSsNP,CRSwNP).
- 分析压力,抗微生物耐药性和病毒性基因,包括等离子体和菌体.
- 在不同患者群体中识别和比较不同的毒性因子基因模式.
主要成果:
- 确定了四种不同的S. aureus毒性因子基因模式:所有分离物和辅助组的共同核心组,包括肠毒素和侵入性毒性因子.
- CRSwNP分离物显示了核心毒性因子集的不完整携带,具有显著的特定基因损失,如scn, icaC和hlgA.
- 在某些毒性因子基因群和患者组之间观察到统计学上显著的关联 (p = 0.001).
结论:
- 黄金杆菌表现出集群的毒性因素,这些因素可能会合作提高细菌的生存,持久性和病原性.
- 观察到的毒性因子基因模式的差异,特别是在CRSwNP分离物中,可能解释了CRS中对抗生素的治疗耐药性和折射性.
- 了解这些毒性因子的动态对于开发针对性疗法对抗慢性鼻炎中持续性金黄色细菌感染至关重要.
相关概念视频
Nose and Nasal Cavity
17.1K
The nose is composed of an observable exterior segment (external nose) and an internal segment within the skull known as the nasal cavity (internal nose). The external nose, visible on the face, consists of a framework of bone and hyaline cartilage enveloped in skin and muscle and lined with a mucous membrane. This structure is supported by the frontal bone, nasal bones, and maxillary bone and is supplemented by a cartilaginous framework comprising the septal nasal cartilage, lateral nasal...
17.1K
Chronic Pharyngitis
16.9K
Chronic pharyngitis refers to persistent inflammation of the pharyngial mucosa.
Etiology
It often arises from persistent viral or bacterial infections affecting sinuses and tonsils.
Additional contributing factors include inadequate dental hygiene, mouth breathing, recurring tonsillitis, allergic rhinitis, laryngopharyngeal reflux, and exposure to smoke, chemicals, and other environmental pollutants. Allergic reactions to pollen, mold, and pet dander, chronic cough, excessive voice usage,...
Etiology
It often arises from persistent viral or bacterial infections affecting sinuses and tonsils.
Additional contributing factors include inadequate dental hygiene, mouth breathing, recurring tonsillitis, allergic rhinitis, laryngopharyngeal reflux, and exposure to smoke, chemicals, and other environmental pollutants. Allergic reactions to pollen, mold, and pet dander, chronic cough, excessive voice usage,...
16.9K
Sputum Studies II: Culture and Sensitivity
2.0K
Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
2.0K
Microbiota of the Respiratory Tract
197
The human respiratory tract, comprising the upper and lower segments, serves as a critical interface with the external environment. The upper respiratory tract (URT)—including the nostrils, sinuses, pharynx, and oropharynx—is heavily colonized by microbes, while the lower respiratory tract (LRT), composed of the larynx, trachea, bronchi, and lungs, was long thought to be sterile. However, recent molecular studies have revealed that the lungs are not devoid of microbes but act more...
197
Staphylococcal Skin Infections
150
Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
150
Clinical Significance of Antibiotic Resistance
115
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
115


