第一次裂变的温度对植入前发育和新生儿活力产生影响
Aliya Stanova1, Galina Kontsevaya1, Alexander Romashchenko1,2
1Federal Research Center Institute of Cytology and Genetics, Siberian Branch of RAS, 630090 Novosibirsk, Russia.
International journal of molecular sciences
|May 7, 2025
概括
早期胚胎发育期间较低的化温度可以增强胚胎的适应潜力,尽管最初的变化. 较高的温度可能导致妊娠失败,表明温度温度.
科学领域:
- 胚胎学 胚胎学
- 发展生物学 发展生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 早期的胚胎发育对环境因素很敏感.
- 化温度影响胚胎形态,细胞分裂和DNA甲基化.
研究的目的:
- 研究不同化温度 (35°C,37°C,39°C) 对早期胚胎发育的影响.
- 评估对形态,细胞分裂速率,DNA甲基化和生存能力的影响.
主要方法:
- 在不同温度 (35°C,37°C,39°C) 下进行体外受精和胚胎化.
- 对芽细胞体大小,细胞分裂速度和DNA5甲基细胞素 (5mC) 水平的分析.
- 在体内通过胚胎转移到受体雌性进行生命能力评估.
主要成果:
- 35°C的化导致了布拉斯托马大小变异性和DNA5mC异质性的增加,胚胎早期死亡率更高,但新生儿活力有所改善.
- 39°C化对体外发育没有显著影响,但增加了妊娠失败率.
- 在37°C的化过程中,大多数参数都产生了中间结果.
结论:
- 在胚胎基因组激活 (ZGA) 过程中温度的降低可能会揭示胚胎的适应潜力.
- 化温度升高并不总是影响胚胎早期发育或生存能力.
- 早期胚胎发育的最佳温度需要进一步研究.
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