一种与低毒性相关的分体病毒和分体病毒与细胞生物之间水平基因转移的重新检查
Ting Ye1,2,3, Han Li1,2, Du Hai1,2
1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
International journal of molecular sciences
|May 7, 2025
概括
毛皮蛋白 (CP) 和依赖RNA的RNA聚合酶 (RdRP) 基因的水平基因转移 (HGT) 从partitiviruses转移到细胞生物中很普遍. 这项研究确定了一种新型分体病毒,SsAPV1,并发现其基因在包括植物和古生物在内的多种物种中集成.
科学领域:
- 病毒学和分子进化
- 基因组学和生物信息学
- 植物病理学和真菌遗传学
背景情况:
- 之前的研究表明,alphapartitivirus外套蛋白 (CP) 基因被整合到植物基因组中.
- 部分病毒和细胞生物之间水平基因转移 (HGT) 的程度在很大程度上是未知的.
- 植物病原体中的低毒性相关病毒为疾病控制策略提供了潜力.
研究的目的:
- 调查涉及Partitiviridae家族病毒的HGT的流行率和范围.
- 识别和描述新型分体病毒及其与宿主基因组的相互作用.
- 探索病毒基因集成在细胞有机体中的进化影响.
主要方法:
- 发现并描述了一种新型的党派病毒,即斯克莱罗提尼亚斯克莱罗蒂诺亚尔法党派病毒1 (SsAPV1).
- 使用partitivirus CP和RNA依赖RNA聚合酶 (RdRP) 序列作为诱来搜索NCBI数据库的生物信息分析.
- 在感染宿主菌株中检测病毒RNA基因组片段,并分析潜在的逆转录酶活性.
主要成果:
- 鉴定了SsAPV1来自Sclerotinia sclerotiorum的低病毒性菌株,在植物基因组中发现了CP同类物,如Helianthus annuus.
- 证明了CP和RdRP基因的广泛HGT来自所有五种Partitiviridae属的植物,动物,真菌,原生动物和古生物.
- 观察到CP基因在植物基因组和RdRP基因在昆虫基因组中的优先整合,有证据表明感染菌株中存在SsAPV1RNA片段.
结论:
- SsAPV1代表了一种与低毒性相关的分体病毒,在真菌疾病管理中具有潜在的应用.
- 参与病毒和细胞生物之间的HGT事件是广泛的,并且已经发生在不同的种群中.
- 这些发现增强了对partitivirus进化,生态及其在细胞生物体基因组进化中的作用的理解,可能涉及病毒逆转录酶活性.
关键词:
在RdRP中使用RdRP.斯克莱洛提尼亚 斯克莱洛提尼亚 斯克莱洛提尼亚 斯克莱洛提尼亚细胞进化的细胞进化.毛皮蛋白质是一种毛皮蛋白质.横向基因转移是指水平基因转移.这是一种低毒性 (hypovirulence).我的病毒病毒病毒病毒病毒.在 partitivirus 中使用.更多相关视频
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