相关实验视频

Updated: May 15, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

在丝虫,Bombyx mori中的BmGDAP2的CRISPR/Cas9中介淘汰:延长寿命和改变基因表达影响发育途径

Chaojun Yuan¹, Zichong Zhou¹, Qifeng Guo¹

¹Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, China.

|May 7, 2025

概括

在丝虫中淘汰类胺诱导的分化相关蛋白2 (GDAP2) 基因显著延长了它们的生命周期. 这是由于对控制自,亡和激素调节的基因的影响造成的.

科学领域:

遗传学遗传学是一个
发展生物学发展生物学
分子生物学分子生物学

背景情况:

氏体诱导的分化相关蛋白2 (GDAP2) 与人类遗传性小脑动有关.
在昆虫中GDAP2的功能在很大程度上仍然未被描述.
在主要的丝虫组织,如头部,表皮,中肠和前丝腺中,GDAP2的表达高.

研究的目的:

为了研究GDAP2在丝虫,Bombyx mori.中的功能.
阐明GDAP2影响昆虫发育和生命周期的分子机制.

主要方法:

使用CRISPR/Cas9基因编辑生成BmGDAP2淘汰赛 (BmGDAP2^KO) 突变物.
对BmGDAP2^KO和野生型丝虫进行比较分析,包括生长,发育和基因表达概况.
对差异表达基因进行了基因本体学 (GO) 和KEGG通路分析.

主要成果:

与野生类型相比,BmGDAP2^KO幼虫的生长周期显著延长,体型缩小.
确定了149个差异表达的基因,GO分析突出了结构/分子活动和催化/结合功能的作用.
凯格分析显示,与过氧化酶活性,激素合成,细胞亡和寿命调节相关的途径的丰富,MAD2L1和Aurka-b的显着下调.

结论:

淘汰BmGDAP2显著延长了Bombyx mori.的生命周期.
这种效应是由参与自,亡和激素调节的基因中断的媒介.
BmGDAP2在调节细胞分裂,细胞亡和潜在的荷尔蒙合成方面发挥着至关重要的作用,影响丝虫的整体发育和寿命.

关键词:

在 GDAP2 中.发展发展发展发展发展.寿命长度寿命长度丝虫是一种丝虫.

更多相关视频

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.