Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Cell shape dynamics during mouse peri-implantation development and embryo dormancy.

Current topics in developmental biology·2026
Same author

Developmental regulation of Erk signaling by mitotic kinases.

Science advances·2026
Same author

Dual role of Oct4 and Sox2 in controlling the developmental capacity and timing of tissue morphogenesis in the embryonic lineage.

Developmental cell·2025
Same author

Pause and play: insights into mammalian embryonic diapause.

Development (Cambridge, England)·2025
Same author

ETMR stem-like state and chemo-resistance are supported by perivascular cells at single-cell resolution.

Nature communications·2025
Same author

3D In Vitro Culture of Early Mouse Embryos and Trophoblast Tissue Explants.

Methods in molecular biology (Clifton, N.J.)·2025

相关实验视频

Updated: May 12, 2025

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
09:14

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Published on: June 14, 2024

703

诱导,维持和退出小鼠胚胎休眠状态的协议.

Rui Chen1, Rui Fan1, Ivan Bedzhov1

  • 1Embryonic Self-Organization Research Group, Max Planck Institute for Molecular Biomedicine, Röntgenstraße 20, 48149 Münster, Germany.

STAR protocols
|May 7, 2025
PubMed
概括

这项研究详细介绍了在小鼠中诱导和维持胚胎隔膜 (发育延迟) 的方案. 研究人员现在可以可靠地暂停和重新激活早期胚胎发育以进行生殖研究.

科学领域:

  • 生殖生物学 生殖生物学
  • 发育生物学 发展生物学
  • 哺乳动物繁殖 哺乳动物繁殖

背景情况:

  • 胚胎断层是一种哺乳动物的生殖策略,涉及暂时暂停胚胎发育.
  • 这种适应允许延迟植入和分娩,使发育与最佳环境条件同步.
  • 了解隙机制对于生殖科学和保护至关重要.

研究的目的:

  • 在小鼠模型中提供一个详细的,逐步的协议,用于诱导和维持胚胎断.
  • 概述了隔离休眠胚胎并触发其重新激活的方法.
  • 促进对哺乳动物生殖适应性的可复制性研究.

主要方法:

  • 在小鼠中通过托莫西芬或卵巢切除来诱导胚胎隔膜.
  • 关于老鼠交配,药物给药和手术的详细程序.
  • 隔离休眠胚胎和随后使用β- Estradiol重新激活.

主要成果:

  • 在使用描述的方法,成功诱导和维持小鼠的胚胎隔膜.
  • 证明了休眠和重新激活胚胎的可靠隔离.
  • 建立一个可复制的协议来研究胚胎断.

结论:

关键词:
细胞生物学 细胞生物学发育生物学是发展生物学.遗传学 是一个遗传学.代谢过程中的代谢.模型生物模型生物干细胞是干细胞.

更多相关视频

Induction of Maternal Immune Activation in Mice at Mid-gestation Stage with Viral Mimic PolyI:C
07:13

Induction of Maternal Immune Activation in Mice at Mid-gestation Stage with Viral Mimic PolyI:C

Published on: March 25, 2016

18.2K
Author Spotlight: Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination
05:36

Author Spotlight: Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination

Published on: July 7, 2023

7.7K

相关实验视频

Last Updated: May 12, 2025

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
09:14

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Published on: June 14, 2024

703
Induction of Maternal Immune Activation in Mice at Mid-gestation Stage with Viral Mimic PolyI:C
07:13

Induction of Maternal Immune Activation in Mice at Mid-gestation Stage with Viral Mimic PolyI:C

Published on: March 25, 2016

18.2K
Author Spotlight: Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination
05:36

Author Spotlight: Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination

Published on: July 7, 2023

7.7K
  • 提出的协议提供了一个标准化的方法来研究小鼠的胚胎隔膜.
  • 这种方法可以进一步研究发展停止和重新激活背后的分子和生理机制.
  • 该议定书支持对生殖策略和辅助生殖的潜在应用的研究.