相关实验视频

Updated: May 12, 2025

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

常规LC-MS/MS方法用于在收获阶段和加工过程中量化番茄中的Alternaria毒素

Léna Dole¹, Noël Durand^1,2, Charlie Poss^1,2

¹Qualisud, Univ Montpellier, CIRAD, Avignon Université, Institut Agro, IRD, Univ de La Réunion, Montpellier, France.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment

|May 7, 2025

概括

一种新的方法量化番茄中有害的Alternaria毒素. 主要毒素的亚酸是热稳定的,在番茄加工残留物中缩.

科学领域:

食品安全和毒理学
分析化学是一种分析化学.
菌类毒理学菌类毒理学

背景情况:

替代物种污染番茄,产生产生健康风险的真菌毒素.
了解这些毒素在食品加工过程中的流行和命运对于消费者安全至关重要.
现有的分析方法对于常规监测来说可能是复杂和昂贵的.

研究的目的:

开发和验证一种具有成本效益的,高性能的液体染色体协同质谱法 (HPLC-MS/MS) 方法,用于量化关键的Alternaria毒素.
评估来自西红的不同Alternaria菌株的毒素生产概况.
研究食品加工对番茄中自然存在的Alternaria毒素的积累和稳定性的影响.

主要方法:

开发和验证HPLC-MS/MS方法来定量替代醇,替代醇单甲基乙烯,亚酸,阿尔特烯,阿尔特毒素I和特索.
从番茄中分离出的各种Alternaria菌株的毒素配置分析.
在自然污染的番茄上进行加工操作的试点规模评估.

主要成果:

开发的HPLC-MS/MS方法实现了低于欧盟建议的量化极限 (LOQ),具有良好的回收率 (85-103%) 和可重复性 (<15%).
十亚酸被确定为由Alternaria菌株产生的主要真菌毒素,并在受污染的西红中发现.
加工操作没有降低亚酸水平,表明其热稳定性. 番茄皮和种子残留物显示污染的程度明显高 (2.6倍) 于纸.

结论:

经过验证的HPLC-MS/MS方法适用于番茄中Alternaria毒素的常规分析,降低了成本和复杂性.
由于其在加工过程中的普遍性和热稳定性,亚酸在番茄污染中是一个主要问题.
番茄加工的副产品 (皮肤,种子) 积累了更高水平的毒素,需要仔细考虑它们的再利用.

关键词:

阿尔特纳里亚毒素的毒素在HPLC-MS/MS中使用.处理处理处理处理在这里,我们可以看到茄,番茄,番茄.

更多相关视频

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

$Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems$ 14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

相关概念视频

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

High-Resolution Mass Spectrometry (HRMS)

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...