奶酪中的新出现的真菌毒素:通过LC-MS/MS对阿弗拉托克辛M,阿弗拉托克和斯特里格马托西斯的同时分析
Maurizio Cossu1, Andrea Sanna1, Giuseppe Mangano1
1Istituto Zooprofilattico Sperimentale della Sardegna "G.Pegreffi", Via Vienna, n.2, 07100 Sassari, Italy.
Molecules (Basel, Switzerland)
|May 7, 2025
概括
这项研究开发了一种可靠的方法来检测乳酪中的 aflatoxin M1 (AFM1),aflatoxicol (AFL) 和sterigmatocystin (STC). 经过验证的技术通过量化这些有害的真菌毒素来确保奶酪的安全性.
科学领域:
- 食品科学 食品科学 食品科学
- 分析化学 分析化学
- 食品安全 食品安全
背景情况:
- 奶酪中的真菌毒素对健康构成重大风险.
- 污染发生在动物料或直接的菌生长中.
- 奶酪可能含有阿弗拉托克辛B1代谢物,如AFM1,AFL和STC.
研究的目的:
- 开发和验证一种可靠的方法,用于同时确定各种奶酪中的AFM1,AFL和STC.
- 评估菌毒素从牛奶转移到奶酪以及它们在奶酪生产过程中的生长.
主要方法:
- 单一液体提取,然后进行免疫亲和系列 (IAC) 清理.
- 使用液体染色学-并联质谱法 (LC-MS/MS) 进行测定.
- 方法根据欧盟官方食品控制法规进行验证.
主要成果:
- 已达到的低量化极限 (LOQ):AFM1的2.0 ng/kg,AFL的5.0 ng/kg,STC的1.0 ng/kg.
- 该方法适用于绵羊,山羊和水牛奶酪.
- 应用方法研究菌毒素转移和生长动态.
结论:
- 本文介绍了一种经过验证,可靠的LC-MS/MS方法,用于同时对奶酪中的真菌毒素进行分析.
- 这种方法支持可靠的食品安全监测和风险评估.
- 对于确保消费者的乳制品安全至关重要.
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