通过水力动力学生成的多层皮肤球形使生物活性成分的体外查和毒性测试成为可能
Zhengkun Chen1, Dianoosh Kalhori1, Faeze Rakhshani1
1Department of Chemistry, University of Toronto, Toronto, ON, Canada.
Science advances
|May 7, 2025
概括
研究人员使用纤维细胞球体涂上角质细胞创建了一个新的皮肤模型. 这种多层球形系统精确地模仿皮肤结构和功能,用于先进的体外试验.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 皮肤病学 皮肤病学
背景情况:
- 多层组织结构对于生理功能至关重要.
- 目前的多细胞球体缺乏定义的多层架构,限制了它们在疾病建模和毒性测试中的使用.
- 在体外重建皮肤的层次结构对于准确的生物评估至关重要.
研究的目的:
- 开发一种以水力动力学为媒介的方法,用于生成多层皮肤球形.
- 在体外模型中重复皮肤的表皮和皮肤层.
- 为查和毒性评估创建一个功能性皮肤模型.
主要方法:
- 纤维细胞球体 (皮肤核心) 的水力动力学介导生成.
- 纤维细胞球体被皮细胞包围,形成一个表皮层.
- 对表皮,皮肤-表皮连接和皮肤的生物标志物表达的评估.
- 评估类似皮肤的屏障特性和功能测试.
主要成果:
- 成功生成了大量的多层皮肤球形,具有明显的皮肤和表皮层.
- 球状体表达了关键的生物标志物,表明了原生皮肤结构.
- 在体外 (in vitro) 证明了类似皮肤的屏障特性和功能反应.
- 查与临床数据相关的维生素协同作用和化学毒性.
结论:
- 开发的以水力动力学为媒介的方法可以精确控制球形细胞分布.
- 这种方法有助于创建多层组织,特别是皮肤的先进体外模型.
- 该模型显示出在疾病建模,药物查和毒理学方面的应用具有前景.
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