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Updated: May 20, 2025

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Published on: April 11, 2019

光触发的CRISPR/Cas12a用于基因组编辑和瘤回归

Hong Liu¹, Jiantong Dong², Renzhi Wu¹

¹State Key Laboratory of Geomicrobiology and Environmental Changes, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, 430074, China.

Angewandte Chemie (International ed. in English)

|May 7, 2025

概括

一种新的光触发CRISPR/Cas12a系统 (LAC12aGE) 能够在细胞和瘤中精确编辑基因. 这个系统的光激活有效地破坏了目标基因,抑制了瘤生长,并在体内促进了亡.

科学领域:

分子生物学分子生物学
基因编辑技术的技术
生物技术是生物技术.

背景情况:

克里斯普尔/Cas12a系统提供精确的基因编辑能力.
在空间和时间上控制CRISPR/Cas12a活动仍然是一个挑战.
光响应系统可以增强基因编辑的时空控制.

研究的目的:

开发一个由照片触发的CRISPR/Cas12a基因编辑系统 (LAC12aGE).
为了证明LAC12aGE在体外和体内基因编辑方面的有效性.
评估LAC12aGE在癌症模型中的治疗潜力.

主要方法:

开发一种对CRISPR/Cas12a具有光响应的子crRNA.
通过光激发的CRISPR/Cas12a机制的激活 (LAC12aGE).
在细胞系和体内瘤模型中应用LAC12aGE进行基因编辑.

主要成果:

在活细胞中,LAC12aGE成功编辑了外源和内源基因.
肝细胞生长因子 (HGF) 基因的淘汰抑制了HepG2细胞的增殖和迁移.
在体内给药LAC12aGE有效抑制了HepG2瘤的生长,诱导了亡和亡.

结论:

照片触发的CRISPR/Cas12a系统提供了对基因编辑的精确时空控制.
LAC12aGE在体外基因编辑应用方面显示出显著的潜力.
作为一种治疗策略,LAC12aGE在体内抑制瘤生长具有前景.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.基因修复基因修复机械设备机械设备机械设备光反应性核酸的核酸.时间空间的时间空间.

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