相关实验视频
Updated: Jun 16, 2025

Ileectomy-induced Bile Overaccumulation in Mouse Intestine
Published on: August 21, 2017
柏林通过调节胆酸通过肠道FXR通过肝肠道循环来改善性结肠炎
Mingyun Chen1, Yifan Wang2, Lin Chen2
1College of Pharmaceutical Sciences, Southwest University, Chongqing, China; Engineering Research Center of Coptis Development and Utilization (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing, China; Chongqing Key Laboratory of New Drug Screening from Traditional Chinese Medicine, College of Pharmaceutical Sciences, Southwest University, Chongqing, China; Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, China.
来自Coptidis Rhizoma的Epiberberine有效地治疗性结肠炎 (UC),通过肠道FXR激活恢复胆酸平衡. 这项研究揭示了通过调节胆酸代谢来管理UC的新型治疗途径.
科学领域:
- 胃肠病学 胃肠病学
- 药理学 药理学是指药理学的学科.
- 分子生物学分子生物学
背景情况:
- 性结肠炎 (UC) 与失调的胆酸代谢有关,其中过多的肠胆酸会使炎症恶化.
- 科普蒂迪斯瘤对UC有治疗前景,但其对胆酸调节的影响尚不清楚.
研究的目的:
- 为了研究epiberberine的治疗作用,一个Coptidis Rhizoma类化合物,在UC.
- 通过肠道Farnesoid X受体 (FXR) 激活来阐明epiberberine在恢复胆酸平衡中的机制.
主要方法:
- 在UC小鼠模型和细胞培养中验证了epiberberine的抗炎作用和FXR激活.
- 使用生物化学和生物物理技术确定了epiberberine-FXR结合相互作用,包括CETSA,SIP,DARTS,IP-MS和ITC.
- 利用分子对接,突变发生和Fxr缺乏的小鼠来确认epiberberine的体内目标和机制.
主要成果:
- 在UC小鼠中,epiberberine激活了肠道FXR,上调胆酸载体 (OSTα,OSTβ,IBABP) 和FGF15分泌,促进胆酸再吸收和抑制胆酸合成.
- 这导致肠道胆酸积累减少,并抑制了炎症标志物 (NF-κB,IL-1β,IL-10,IL-6).
- 在MET265和ARG331中,epiberberine直接与FXR结合 (KD=2.04μmol/l);FXR缺乏消除了epiberberine对胆汁酸和UC的有益作用.
结论:
- UC的特点是FXR的抑制和胆汁酸的异常积累.
- 乙柏林通过激活叶FXR来改善UC,从而调节胆汁酸输肝循环,并降低肠道胆汁酸水平.
- 这项研究为Coptidis Rhizoma类化合物的作用提供了新的视角,通过胆酸平衡调节来激活UC治疗的肠道FXR.
更多相关视频
08:58Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
Published on: January 5, 2017
08:42Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport
Published on: November 27, 2016
相关概念视频
Drugs for Treatment of Ulcerative Colitis in IBD
Inflammatory Bowel Disease IV: Pharmacological Management
Pharmacologic...
Inflammatory Bowel Disease I: Ulcerative Colitis
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Chronic Bowel Disorders: Introduction
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
Drugs for Treatment of Diarrhea-Predominant IBS
Two specific drugs used in the treatment are alosetron (Lotronex) and eluxadoline (Viberzi). Alosetron, a 5-HT3 antagonist, works by slowing the movement of stools in the gut, reducing bowel...
Drugs for Peptic Ulcer Disease: Prostaglandin Analogs as Mucosal Protective Agents
Non-steroidal anti-inflammatory drugs (NSAIDs) can induce peptic ulcers by inhibiting cyclooxygenase, decreasing...