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手术/麻醉可能会导致单细胞促进瘤发育

Yang-Yang Wang¹, Rui-Lou Zhu¹, En-Qiang Chang¹

¹Department of Anesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, 450003, Henan, China.

Molecular medicine (Cambridge, Mass.)

|May 7, 2025

概括

大型手术会影响免疫细胞,T细胞恢复得很快,但调节性T细胞 (Tregs) 和单细胞显示长时间的功能障碍. 单细胞变化可能会在手术后促进瘤的发展.

科学领域:

免疫学免疫学免疫学
手术瘤学手术瘤学
细胞生物学细胞生物学

背景情况:

大型手术引发显著的外科手术期间免疫反应,影响患者的预后.
了解手术/麻醉对免疫细胞的影响对于患者的治疗结果至关重要.

研究的目的:

通过单细胞测序,研究手术和麻醉对外周血液单核细胞 (PBMC) 的影响.
分析在外科手术期间免疫细胞群和功能变化.

主要方法:

对4名患者之前发表的单细胞测序数据的分析.
从手术前和手术后0,24和48小时采集的血液样本中分离PBMC.
使用R包 (Seurat,scSTAR) 进行分析和RT-PCR用于基因表达验证.

主要成果:

CD4+,CD8+T细胞功能迅速恢复;然而,Treg功能在术后48小时内仍然受损.
非古典单细胞被手术显著影响,48小时内没有恢复的迹象.
基因表达分析显示,手术后瘤患者的MDM2和SESN1水平增加.

结论:

手术/麻醉对CD4+和CD8+T细胞的影响最小,但持续改变Treg功能.
单细胞受到显著影响,这表明在术后促进瘤发育方面可能发挥作用.

关键词:

总麻醉是一种全身麻醉.单细胞 (Monocytes) 是一种单细胞.这就是PBMCs.这就是ScRNA-seqq.瘤的发展瘤的发展

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