在油性酵母Rhodotorula toruloides中的乙烯基醇代谢
Vittorio Giorgio Senatore1, Alīna Reķēna2, Valeria Mapelli1
1Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza Della Scienza 2, 20126, Milan, Italy.
红色酵母Rhodotorula toruloides可以高效地将乙烯基醇 (EG) 转化为甘油酸 (GA). 这一过程还提高了西洛斯的利用率,为生物炼油厂的废物利用提供了双重好处.
科学领域:
- 生物技术和工业微生物学
- 生物化学工程 生物化学工程
- 可持续化学 可持续化学
背景情况:
- 农业食品废弃物是一个重大挑战,包括纤维素生物质和塑料,如聚乙烯四甲酸盐 (PET).
- 酶解水解和微生物发酵为将各种废物流转化为有价值产品提供了上循环的途径.
- 油性酵母Rhodotorula toruloides以其代谢的多功能性和生物处理的潜力而闻名.
研究的目的:
- 为了研究由Rhodotorula toruloides的乙烯糖醇 (EG) 的代谢.
- 评估EG共同代谢对葡萄糖和西洛斯利用的影响.
- 探索R. toruloides从废物中生产甘油酸 (GA) 和微生物油的潜力.
主要方法:
- 进行了批量生物反应器实验,以评估R. toruloides中的EG催化作用.
- 生理学研究的重点是EG代谢与葡萄糖,氧化糖和混合碳来源.
- 使用in silico分析,提出EG氧化的代谢途径.
主要成果:
- 在所有测试条件下,Rhodotorula toruloides将乙烯基醇 (EG) 代谢为甘油酸 (GA).
- 在葡萄糖的存在下,从EG获得了100%的GA分子产量,这是酵母的新发现.
- 消耗EG改善了西洛斯生物处理,可能通过再生NAD和减轻辅因子失衡.
- 与葡萄糖的EG代谢发生在限后,与没有EG的对照相比没有显著差异.
结论:
- 罗多托鲁拉托鲁洛伊德 (Rhodotorula toruloides) 显示出从乙烯糖醇 (EG) 中生物制造甘油酸 (GA) 的巨大潜力.
- EG与林氏纤维素糖 (如西洛) 的共同代谢可以提高生物过程的效率.
- 这种酵母为塑料和生物质废弃物的同时价值化为有价值的化学品和微生物油提供了一个有希望的平台.
更多相关视频
14:53Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
14:42Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
相关概念视频
Fates of Pyruvate
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
Fermentation
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Glycolysis: Preparatory Phase
Energy-requiring Steps of Glycolysis
What is Glycolysis?
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
Energy-releasing Steps of Glycolysis
The first energy-releasing step—the 6th step of glycolysis...
