建立一个高通量缩减克隆选平台,用于加强CHO细胞的料批培养
Haiyan Luo1, Shuai Wang1, Collin Chong1
1WuXi Biologics, Cell Line Development, Mashan, Wuxi, China.
Biotechnology letters
|May 8, 2025
概括
一个新的强化养批量 (IFB) 克隆查平台有效地识别高表达的CHO细胞系. 该平台显著提高了抗体标位,为生物制药生产提供了有效的替代方案.
科学领域:
- 生物技术是生物技术.
- 生物工艺工程 生物工艺工程
- 细胞线发展发展 细胞线发展
背景情况:
- 强化料批次 (IFB) 工艺对于高效的生物制药生产至关重要.
- 选择高表达性克隆对于最大限度地提高IFB培养的生产力至关重要.
- 现有的选方法可能不适合IFB策略.
研究的目的:
- 开发一个规模缩小的克隆选平台,为强化料批次 (IFB) 过程量身定制.
- 为了能够有效地识别与生物反应器中IFB培养条件相容的高表达克隆.
主要方法:
- 使用三种单克隆抗体 (mAbs) 开发和验证IFB特定的克隆查平台.
- 利用自旋管和AMBR250生物反应器系统进行克隆选.
- 优化了这一过程,添加了aurintricarboxylic acid (ATA) 和uridine.
主要成果:
- IFB平台的平均标位为8g/L,最高为9.6g/L.
- 旋转管和AMBR250系统都有效选了适合IFB的克隆,保持了类似的细胞活力和乳酸.
- 使用ATA和尿素的过程优化进一步增加了表达,达到12.2g/L的峰值标位.
结论:
- 开发的IFB流程特定的克隆选平台是工业应用的有效替代方案.
- 该平台提高了选高表达CHO细胞系用于IFB生产的效率和有效性.
- 它促进了优质克隆的选择,以改善生物制药制造业的结果.
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