在"Acetobacterium dehalogenans"中出现了意想不到的16S rRNA异质性,并将其重新归类为"Acetobacterium malicum"亚种. 脱基子 脱基子的亚种 没有了,没有了
Stefan Spring1, Jacqueline Wolf2, Sarah Kirstein2
1Department of Microorganisms, Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
概括
一种新型细菌,以前命名为"Acetobacterium dehalogenans"MCT,被重新归类为一个亚种的Acetobacterium malicum. 这次重新分类是基于全基因组测序和化学分类学分析,确定了A. malicum亚种. 脱素人亚种 脱素人亚种. 在新的一年里.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 基因组学就是基因组学.
背景情况:
- 菌株MCT,是一种严格无氧的同类乙原菌,由于其利用甲基化物获取能量的能力,暂时被确定为"Acetobacterium dehalogenans".
- 16S rRNA基因的直接测序是具有挑战性的,因为该菌株的五个rRNA操作子之间存在显著的异质性.
研究的目的:
- 为了解决菌株MCT的分类学分类.
- 调查菌株MCT和相关的Acetobacterium物种之间的遗传学和化学分类学关系.
主要方法:
- 进行了全基因组测序,以分析rRNA操作子并比较基因组.
- 计算了数字DNA-DNA杂交 (dDDH) 和平均核酸标识 (ANI).
- 进行了化学分类学分析,包括脂肪酸和极性脂质分析.
主要成果:
- 全基因组测序揭示了菌株MCT的rRNA操作子中的显著异质性 (超过1.4%的序列差异).
- 基因组比较显示,菌株MCT与Acetobacterium malicum MuME1T最密切相关,dDDH为71.9%,ANI为96.59%,表明它们属于同一物种.
- 菌株MCT在甲醇和葡萄糖的利用上与A. malicum不同,但在酸盐的利用上是相同的. 观察到明显的脂肪酸和极性脂质配置文件.
结论:
- 菌株"Acetobacterium dehalogenans"MCT被重新分类为一个新的亚种,A. malicum*亚种. *脱素*亚种 *脱素*亚种 在新的一年里.
- 这一分类学修订确立了*A. malicum*亚种. *malicum* 小品种 *malicum* 小品种 nov.与MuME1T作为类型菌株.
- 这些发现强调了全面的基因组和化学分类学分析对于准确的细菌分类的重要性.
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