生物信息学模型对预测血管新生转化控制的价值
Michal Shaposhnikov1,2, Juilee Thakar3,4, Bradford C Berk1,2
1Department of Cellular and Molecular Pharmacology and Physiology (M.S., B.C.B.), University of Rochester School of Medicine and Dentistry, NY.
Circulation research
|May 8, 2025
概括
本综述强调了用户友好的生物信息学工具,用于研究血管生成中的转化调节,弥合了专家和研究人员之间的差距. 可访问的平台使科学家能够发现新的转化调节剂,加速生物学和医学方面的发现.
科学领域:
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
- 遗传学 遗传学 是一个
背景情况:
- 血管新生,新血管的形成,对于生理和病理过程至关重要.
- 血管生成的转录调节 (例如,由HIF-1α和VEGF) 已被理解,但转化调节尚未得到充分研究.
- 生物信息学专业知识与更广泛的研究可访问性之间存在差距,阻碍了预测工具的使用.
研究的目的:
- 审查用户友好的生物信息学工具在阐明血管生成中的转化调节中的作用.
- 突出可访问的平台来分析RNA结构,微RNA,lncRNA和RNA结合蛋白.
- 弥合生物信息学知识差距,并使研究人员能够利用这些工具.
主要方法:
- 审查关于生物信息学工具的现有文献,用于翻译调节.
- 讨论使用户友好的平台 (例如,RNAstructure,H-iRIDE,RBPSuite) 与Web浏览器接口.
- 重点是分析RNA动机和RNA蛋白相互作用,用于预测建模.
主要成果:
- 易于使用的生物信息学工具民主化了对用于翻译监管的预测分析的访问.
- 这些工具可以研究复杂的RNA相互作用和调节机制.
- 可访问的平台有助于产生假设,并降低实验成本.
结论:
- 生物信息学工具对于理解血管生成中的转化控制具有变革性.
- 可访问的平台赋予跨学科的研究人员权力,超越血管新生,扩展到临床护理.
- 弥合生物信息学知识差距可以加速科学发现和创新.
相关概念视频
Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
Mechanism of Angiogenesis
5.0K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.0K
Regulation of Expression Occurs at Multiple Steps
21.9K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
21.9K
Leaky Scanning
5.0K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.0K
Regulation of Expression at Multiple Steps
848
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
848
Regulated mRNA Transport
6.2K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.2K


