miR-301a-5p调节IKKβ/NF-κB轴和巨细胞两极分化,以加速皮肤伤口愈合
Yun Li1, Zhe Fu1, Chengjie Deng1
1Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, Yunnan 650500, China.
概括
微RNA miR-301a-5p通过控制炎症和促进组织修复来调节伤口愈合. 它的过度表达增强了皮肤再生和原沉积,为伤口愈合提供了潜在的治疗策略.
科学领域:
- 分子生物学分子生物学
- 再生医学是一种再生医学.
- 皮肤病学 皮肤病学
背景情况:
- 伤口愈合涉及复杂的阶段,微RNA (miRNA) 失调与愈合受损有关.
- 在伤口愈合中miRNAs的精确作用和机制需要进一步阐明.
研究的目的:
- 在伤口愈合期间调查miRNA表达模式.
- 描述miR-301a-5p在伤口愈合和阶段过渡中的功能和机制.
主要方法:
- 在伤口愈合过程中检测和描述miRNA表达特征.
- 评估miR-301a-5p对巨细胞行为和炎症反应的影响.
- 涉及基因鉴定 (IKKβ) 和途径分析 (NF-κB) 的机制研究.
- 在体内实验中,使用大小皮肤伤口的小鼠模型进行了实验.
主要成果:
- 确定了miR-301a-5p的表达动态,在从炎症阶段过渡到增殖阶段时达到顶峰.
- miR-301a-5p促进了巨细胞的增殖和迁移,同时通过调节IL-10和TGF-β来抑制过度炎症.
- 从机制上讲,miR-301a-5p针对IKKβ,调节NF-κB通路和M1-M2巨细胞两极分化.
- 过度表达miR-301a-5p显著加快了小鼠的全厚皮肤伤口再生.
结论:
- 在伤口愈合过程中,miR-301a-5p作为一种新的调节剂,特别是在调节炎症到增殖阶段的过渡过程中.
- miR-301a-5p通过影响NF-κB信号传递和巨细胞极化来促进表皮再生和原沉积.
- 这项研究确定miR-301a-5p作为促进伤口愈合的潜在治疗剂.
更多相关视频
07:55A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
Published on: January 7, 2020
7.3K
06:36Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
Published on: August 21, 2020
11.8K
相关概念视频
NF-κB-dependent Signaling Pathway
7.2K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.2K
Inflammatory Response
1.6K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
1.6K
Inflammation
51.8K
Overview
51.8K
MicroRNAs
21.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.1K
The JAK-STAT Signaling Pathway
8.5K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.5K
Phases of Wound Repair
5.7K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
5.7K
