范B转位子分析检测了抗万科素的Enterococcus faecium水平基因转移:描述了两个爆发的情况
Heleen J Schuster1, Rosa van Mansfeld1, Wil A van der Reijden2
1Medical Microbiology and Infection Control, Amsterdam UMC location University of Amsterdam, Amsterdam, The Netherlands; Amsterdam institute for Infection and Immunity, Amsterdam UMC location University of Amsterdam, Amsterdam, The Netherlands.
The Journal of hospital infection
|May 8, 2025
概括
与全基因组MLST (wgMLST) 一起分析菌素耐药基因,可以改善对菌素耐药Enterococcus faecium (VRE) 疫情的追踪. 这种方法检测了标准类型错过的水平基因转移,增强了疫情调查.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染病流行病学 传染病流行病学
背景情况:
- 抗万科素的Enterococcus faecium (VRE) 爆发是一个重大的全球医疗保健挑战.
- 目前的类型化方法,如全基因组多位置序列类型化 (wgMLST),可以通过VRE菌株的低基因组变异来限制.
研究的目的:
- 开发一种新的生物信息管道,用于分析万科米辛耐药性基因,特别是vanB基因.
- 将这种新方法与wgMLST集成,用于实时调查VRE爆发.
主要方法:
- 创建了一个生物信息学管道,以比较携带vanB抗性基因的转子子序列.
- 该管道与wgMLST一起应用,以调查两个并发的VRE爆发.
- 来自样本的多个殖民地和对菌素敏感的分离物被测序以进行比较.
主要成果:
- wgMLST确定了属于两个不同的VRE爆发的菌株.
- 分析显示,具有不同的wgMLST配置文件的菌株中具有相同的vanB转体,表明水平基因转移.
- 转子子体分析确定了仅由wgMLST错过的潜在疫情传播.
结论:
- 实时转位子分析为vanB-VRE疫情调查提供了关键的补充数据.
- 这种方法有效地检测到横向基因转移,这种现象通常被传统的排版所忽视.
- 转位子测序是对wgMLST进行有价值的增强,用于管理vanB-VRE爆发.
相关概念视频
01:33Bacterial Transformation
54.9K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
54.9K
02:18Types of Genetic Transfer Between Organisms
26.8K
Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
26.8K
02:13Overview of Transposition and Recombination
15.0K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.0K
00:57Antibiotic Selection
51.9K
Overview
51.9K
03:21Genome Size and the Evolution of New Genes
7.8K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
7.8K
00:57Viral Recombination
23.1K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
23.1K