尼古丁胺胺单核酸在代谢工程中产生Saccharomyces cerevisiae
Rhudith B Cabulong1, Won-Gyun Oh1, Tahseena Naaz1
1Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
ACS synthetic biology
|May 9, 2025
概括
这项研究优化了尼古丁胺胺单核酸 (NMN) 的微生物生产,这是一个关键的NAD+前体. 在酵母中使用构成性促进剂显著提高了NMN和NAD+水平,从尼古丁胺胺获得了创纪录的产量.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 酵母发酵 酵母发酵的方法
背景情况:
- 尼古丁胺胺氨基二核酸 (NAD+) 对于细胞能量和新陈代谢至关重要.
- 尼古丁胺胺单核酸 (NMN) 是一种具有抗衰老潜力的直接NAD+前体.
- 微生物NMN生产为化学合成提供了一个可持续的替代方案.
研究的目的:
- 为了优化Saccharomyces cerevisiae中的NMN生产.
- 为了比较构成性 (TEF1) 与诱导性 (GAL1) 促进剂对于NMN生物合成的疗效.
- 通过基因工程和媒体优化来增强细胞内NMN和NAD+产量.
主要方法:
- 改造的酵母菌株 (S. cerevisiae BY4742) 以表达人体尼古丁胺酸转移酶 (h-NAMPT) 和酵母PRS基因.
- 使用TEF1 (构成性) 和GAL1 (诱导性) 促进剂进行基因表达.
- 使用丰富的R-SD介质优化发酵条件.
主要成果:
- 构成性TEF1促进剂产生的细胞内NMN和NAD+比GAL1促进剂更高.
- 在scTEF2g菌株中,从尼古丁胺在瓶发酵中的尼古丁胺中获得了创纪录的151.71mg/L细胞内NMN.
- 与对照菌株相比,NMN产量增加了3倍.
结论:
- 促进者选择对于最大限度地提高酵母中的NMN产量至关重要.
- 优化的酵母发酵为NMN生物合成提供了一个可行的平台.
- 这项研究实现了从尼古丁胺胺的重组酵母中报告的最高细胞内NMN产量.
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