对益生菌酵母Saccharomyces boulardii中高效尿酸降解活性进行系统工程
Wenzhuo Wang1,2, Lei Pan2,3, Huansha He2
1School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Panyu District, Guangzhou, Guangdong 510006, PR China.
ACS synthetic biology
|May 9, 2025
概括
工程酵母益生菌可以有效地分解尿酸,为管理高尿血和诸如痛风等相关疾病提供了一个有希望的新策略. 这项研究开发了一种高效的途径,用于酵母中的尿酸代谢.
科学领域:
- 代谢工程是代谢工程.
- 微生物学 微生物学
背景情况:
- 超尿血症是一种常见的代谢疾病,与痛风,脏和心血管问题有关.
- 目前针对高尿血症的益生菌方法在效率和性能方面面临局限性.
研究的目的:
- 在酵母中设计一种高效的尿酸代谢途径,用于治疗超尿血症.
- 开发一种具有增强尿酸降解能力的复合益生菌株.
主要方法:
- 鉴定并优化了一种来自*Vibrio vulnificus*的尿酶,用于*S. cerevisiae**中的尿酸降解.
- 通过嵌合体结构改善尿酸输送器UAPA的稳定性和表达,以增强尿酸的进口.
- 组装了具有功能组件的构成性促进剂,以创建和选各种代谢途径.
- 将优化的途径集成到益生菌*S. boulardii*基因组中.
主要成果:
- 在工程*S. cerevisiae*中达到365.32±20.54μmol/h/OD的高尿酸降解活性.
- 在高密度发酵下,成功地转移并维持了 *S. boulardii* 中的途径的活动.
- 证明了*S. boulardii*作为重组益生菌的潜力,用于治疗高尿血症.
结论:
- 开发了一种高潜力的酵母益生菌菌株,用于治疗高尿素血.
- 展示了工程益生菌用于代谢疾病治疗的可行性.
- 突出了酵母中途径建设和优化的系统策略.
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