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对自然样本中布罗迪法库姆的生物可用性和菌辅助的光谱定量研究

Ganesh Suku¹, Anupama Vijayan¹, John Prakash¹

¹Department of Chemistry, School of Basic and Applied Sciences, Central University of Tamil Nadu, Thiruvarur, 610 005, India. johnprakash@cutn.ac.in.

Analytical methods : advancing methods and applications

|May 9, 2025

概括

水性湿酸增强了brodifacoum的溶解性,并使敏感的微粒辅助光量化. 这种方法可以准确地检测纳米分子水平的粉,这对于环境和健康监测至关重要.

科学领域:

环境化学环境化学
分析化学分析化学
生物化学生物化学

背景情况:

布罗迪法库姆 (BDF) 是一种具有较低水溶性的强效抗凝剂,阻碍其检测,并带来环境/健康风险.
酸 (HA) 作为伪表面活性剂,可能增加BDF的溶解性和生物可用性.
由于其毒性和广泛使用,准确量化BDF是必不可少的.

研究的目的:

为了探索由水性酸 (HA) 溶解布罗迪法 (BDF) 的溶解.
为BDF量化开发一种敏感的菌辅助光方法.
评估BDF与牛血清白蛋白 (BSA) 的相互作用,以了解生物可用性影响.

主要方法:

在水性HA中确定BDF的溶解效率.
用BSA进行光谱分析 (光) 和结合研究.
微粒的表征 (DLS,共聚焦显微镜) 和基于光的BDF量化.
使用高性能液态染色学 (HPLC) 验证方法.

主要成果:

水性HA显著提高了BDF溶解度 (2.3000 ± 0.0010毫克/小时-1).
BDF对BSA表现出强烈的结合亲和力 (K = 391,000 ± 3.03 L mol-1,n=1),与疏水域IIA.相互作用.
微粒封装增强了BDF光,使得在纳米分子水平 (LOD=62nM,LOQ=208nM) 的量化具有高线性 (R²=0.994).
该方法在HPLC验证中显示出高可靠性 (恢复率~100%,RMSEP=0.1001).

结论:

水性酸有效地溶解了brodifacoum.
微粒辅助光为BDF量化提供了一种敏感且可靠的方法.
了解BDF蛋白相互作用对于评估与增加生物可用性相关的健康风险至关重要.

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