Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Fixation and Sectioning01:03

Fixation and Sectioning

4.1K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
4.1K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.2K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.2K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

5.3K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
5.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Machine Learning-Based Prediction of Polymer Chemical Resistance to Organic Solvents.

ACS omega·2026
Same author

Management of TAVI Candidates With Metal Allergy: Minimally Invasive Aortic Valve Replacement With a Metal-Free Bioprosthesis.

Annals of thoracic surgery short reports·2026
Same author

Formula-Based Relocation of the Papillary Muscle in Patients With Ischemic Mitral Regurgitation.

Annals of thoracic surgery short reports·2026
Same author

Cardiac damage stages at follow-up and subsequent clinical outcomes in patients with severe aortic stenosis.

Cardiovascular intervention and therapeutics·2026
Same author

Surgical Management of Primary and Metastatic Cardiac Malignancies: A 20-Year Single-Center Experience.

Annals of thoracic and cardiovascular surgery : official journal of the Association of Thoracic and Cardiovascular Surgeons of Asia·2026
Same author

Changes in Demographics, Initial Treatment Strategies, and Clinical Outcomes of Severe Aortic Stenosis in the Pre- and Post-TAVR Era in Japan.

Circulation. Population health and outcomes·2026
Same journal

Incremental prognostic value of the hemoglobin, albumin, lymphocyte, and platelet score and b-type natriuretic peptide for 30-day mortality after elective on-pump coronary artery bypass grafting.

Interdisciplinary cardiovascular and thoracic surgery·2026
Same journal

Giant left anterior descending artery aneurysm with an Unusual direct continuity to aortic arch.

Interdisciplinary cardiovascular and thoracic surgery·2026
Same journal

Postoperative c-reactive protein resolution patterns in patients with and without postoperative atrial fibrillation after cardiac surgery.

Interdisciplinary cardiovascular and thoracic surgery·2026
Same journal

Robotic-assisted lobectomy in non-intubated patient for early stage non-small-cell lung cancer treatment.

Interdisciplinary cardiovascular and thoracic surgery·2026
Same journal

The Ross operation in children-what is the long-term fate of the autograft?

Interdisciplinary cardiovascular and thoracic surgery·2026
Same journal

Totally endoscopic mitral valve repair for mitral regurgitation in double-orifice mitral valve.

Interdisciplinary cardiovascular and thoracic surgery·2026
查看所有相关文章

相关实验视频

Updated: May 12, 2025

Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel
08:07

Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel

Published on: December 23, 2022

6.7K

在Frozenix中期形态变化.

Takanobu Kimura1, Hiroshi Tsuneyoshi1, Shuji Setozaki1

  • 1Department of Cardiovascular Surgery, Shizuoka Prefecture General Hospital, 4-27-1 Kita-ando, Aoi, Shizuoka, Shizuoka, Japan.

Interdisciplinary cardiovascular and thoracic surgery
|May 9, 2025
PubMed
概括
此摘要是机器生成的。

冷的大象干支架在手术后可能会扩大,可能导致并发症. 仔细的尺寸和长期成像对于管理这些大动脉门修复至关重要.

关键词:
大动脉动脉瘤的发生.大动脉切割剖析.大动脉外科手术冰的大象尾巴被结了形态变化 形态变化

更多相关视频

Flash Freezing and Cryosectioning E12.5 Mouse Brain
28:32

Flash Freezing and Cryosectioning E12.5 Mouse Brain

Published on: May 28, 2007

42.6K
Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy
12:52

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Published on: October 13, 2014

16.3K

相关实验视频

Last Updated: May 12, 2025

Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel
08:07

Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel

Published on: December 23, 2022

6.7K
Flash Freezing and Cryosectioning E12.5 Mouse Brain
28:32

Flash Freezing and Cryosectioning E12.5 Mouse Brain

Published on: May 28, 2007

42.6K
Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy
12:52

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Published on: October 13, 2014

16.3K

科学领域:

  • 心血管外科心血管外科
  • 生物医学工程 生物医学工程
  • 放射学 放射学是一门学科.

背景情况:

  • 冷大象干 (FET) 技术是对大动脉弧动脉瘤和剖析的标准治疗方法.
  • 潜在的辐射力和FET支架的弹可以导致形态变化和支架诱导的新进入 (SINE).

研究的目的:

  • 评估FET支架安置中的术后形态变化.
  • 为FET支架行为提供生物力学见解.

主要方法:

  • 对107名接受FET安置 (Frozenix) 的患者进行了回顾性分析.
  • 通过计算机断层扫描 (CT) 评估临床结果和形态变化.
  • 测量远距离直径和支架角度以量化变化.

主要成果:

  • 三十天死亡率为3%;所有原因死亡率为9.69/100患者年.
  • 支架诱导的新进入 (SINE) 发生在7%的患者中.
  • 在所有95名被评估的患者中观察到距离直径扩大,其中62%超过原始支架大小;剖析显示扩张大于动脉瘤.
  • 在所有33名被评估的患者中,支架角都增加了,在3年内逐渐发生变化.

结论:

  • 手术后的远距离直径扩大和渐进的支架角度变化是FET安置后的重要发现.
  • 这些形态变化需要在支架大小和手术规划过程中仔细考虑.
  • 建议进行长期CT随访,以监测FET行为.